Glycosphingolipid Analysis Service

Glycosphingolipids (GPLs) are amphipathic biomolecules with a ceramide skeleton and a polysaccharide head, extensively present in cell membranes. They regulate cell behavior and are pivotal in processes such as cellular communication, disease pathogenesis, and cellular recognition. Alterations in GPL levels are associated with neurodegenerative disorders, metabolic imbalances, and certain cancers, highlighting their significance in disease diagnostics and therapeutics. Accurate GPL analysis is vital for both foundational research and clinical applications.

 



Yang Z, et al. Arthritis Res Ther. 2015.

Figure1. Altered Membrane Lipids in Lupus T Cells

 

Mass spectrometry, renowned for its sensitivity and high resolution, is the preferred method for analyzing GPLs. Coupled with High-Performance Liquid Chromatography (HPLC), it enables detailed structural identification and quantification, providing extensive data on their glycan and lipid components.

 

Services at MtoZ Biolabs

At MtoZ Biolabs, we leverage a state-of-the-art mass spectrometry platform and extensive expertise to deliver professional GPL analysis services. Our workflows include:

1. Sample Pre-treatment: Extracting and purifying GPLs from diverse biological samples, utilizing ceramidase to release glycans.

2. Methylation Enhancement: Treating GPLs with methylation to improve sample volatility and mass spectrometric sensitivity.

3. Chromatographic and Mass Spectrometric Profiling: Employing 2AB labeling with UPLC-HILIC-FLD for efficient analysis, followed by detailed mass spec analysis using MALDI-TOF MS and ESI-MS.

4. Structural Elucidation: Using MS/MS to analyze structures based on predicted fragmentation patterns and database comparisons.

 

Additional services include:

1. Quantitative and qualitative GPL analyses.

2. Comprehensive structural elucidation of GPLs.

3. Research on GPL-protein interactions.

 

Sample Submission Requirements

We accept various sample types,  including but not limited to tissue samples(1.0-500 mg), cell samples (≥1×10^6 cells), and biological fluids (1.0-100 µL).

 

MtoZ Biolabs adheres to ICH Q6B standards to ensure thorough characterization of glycoprotein glycosylation. Regardless of the complexity or specificity of your research needs, we provide customized project designs to meet them. If interested in our GPL analysis services, please contact us for more information or to submit samples for evaluation.