Glycosylation Site Analysis Service

Glycosylation, a key post-translational modification, entails the covalent attachment of carbohydrate moieties to specific amino acid residues, significantly influencing protein functions such as folding, stability, and interactions with other biomolecules. Consequently, the comprehensive study of glycosylation sites and glycan structures is crucial for uncovering disease mechanisms, identifying novel biomarkers, and developing innovative therapeutic strategies, particularly in the field of biopharmaceutical research, where glycosylation provides critical insights into the functional roles of proteins.

 

 MtoZ Biolabs offers an extensive Glycosylation Site Analysis Service designed to support researchers in elucidating the complex roles of glycosylation in health and disease.

 

Analysis Workflow

 



 

Services at MtoZ Biolabs

1. Site-Specific Glycosylation Analysis

Utilizing specific enzymatic processes (primarily trypsin) coupled with glycopeptide isolation and mass spectrometric evaluation to pinpoint glycosylation sites.

 

2. Comprehensive Glycosylation Coverage

Employing broad-spectrum proteases, including both exo and endo types, allows us to overcome traditional method limitations, enhancing glycosylation site detection across proteins.

 



Figure 1. Glycosylation Site Analysis

 

Service Advantages

1. Versatile Enzymatic Approaches: We deploy both targeted and broad-spectrum enzymatic methods to liberate glycopeptides effectively from various proteins.

 

2. Advanced Spectrometric Techniques: Using technologies such as MALDI-TOF MS and nano-LC-ESI-MS/MS, we provide meticulous molecular identifications and in-depth structural analyses of glycopeptides.

 

3. In-Depth Data Analysis: Our approach integrates technologies like HPAEC-PAD and HILIC-FLD, supporting comprehensive quantification and analysis of glycosylation patterns.

 

Sample Submission Requirements

 



For more sample details, please consult our technical team.

 

Applications

This service supports numerous critical functions within the biopharmaceutical sector:

1. Drug Development

Ensuring glycosylation consistency and completeness in drug formulations.

 

2. Glycosylation Profiling

Defining and quantifying various glycosylation site types and their prevalence.

 

3. Glycan Evaluation

Assessing the structure and distribution of glycans to inform therapeutic strategies.

 

4. Process Optimization

Aiding in refining production techniques to boost the quality of pharmaceutical products.

 

MtoZ Biolabs is dedicated to delivering accurate, reliable glycosylation analyses, enhancing drug development and assessment in the competitive pharmaceutical market. Our team, composed of seasoned experts, is always ready to provide technical support and tailored solutions to meet your specific research needs.