Histone Modification Analysis Service

Histones are vital nuclear proteins that bind to DNA and regulate gene expression. Their post-translational modifications (PTMs), such as acetylation, methylation, and phosphorylation, play pivotal roles in cell signaling and gene regulation, crucial for understanding biological complexities and disease progression, including cancer. Mass spectrometry, renowned for its sensitivity and resolution, is the method of choice for histone PTM analysis. This technique, enhanced by rapid scanning and precise measurement capabilities, allows for detailed quantitative analyses of protein expression and modification differences.

 



Fallah, M. S. et al. Front Genet. 2021.

Figure1. Histone Methylation Regulates Gene Expression

 

Services at MtoZ Biolabs

At MtoZ Biolabs, we utilize cutting-edge mass spectrometers and Nano-LC systems to offer exhaustive histone modification analysis services, overseeing all aspects from sample reception to report generation. Clients need only define their experimental goals and provide samples; our team of experts handles all subsequent analytical procedures. To enhance the efficiency of trypsin digestion and minimize the loss of small hydrophilic peptides in MS detection, we apply chemical derivatization to increase peptide hydrophobicity, thus boosting both sensitivity and accuracy. Our multi-enzymatic digestion approach further enhances peptide map coverage, enabling the identification of additional PTM sites.

 

Analysis Workflow

1. Isolation and Purification: We employ advanced separation technologies to ensure histones are isolated with high purity, essential for accurate analyses.

2. Multi-Enzyme Digestion: This technique enhances peptide coverage and increases the detectability of PTM sites.

3. High-Performance LC-MS/MS: This integration of advanced chromatography and mass spectrometry techniques ensures high sensitivity and throughput in detecting protein modifications.

4. Bioinformatics Analysis: We utilize sophisticated bioinformatics tools to conduct thorough analyses, verifying the accuracy and applicability of our findings.

 

Applications

1. Comprehensive Analysis: We perform extensive identification and quantification of histone PTMs, encompassing acetylation, methylation, and phosphorylation.

2. Precise Mapping: Our state-of-the-art mass spectrometry accurately locates and identifies modification types and sites.

3. Research and Development Support: Our analyses aid in exploring the links between histone modifications and disease, enhancing research into disease mechanisms and pharmaceutical development.

 

Sample Submission Requirements

For optimal results, MtoZ Biolabs specifies the following requirements for sample submissions:

Plant Tissue: 400mg

Animal Tissue: >2g

Cell Samples: 1X10^8 cells

Urine: 10ml

Blood and Serum: Blood >1mL, Serum >0.5mL

Protein Samples: >2mg

Yeasts/Microorganisms: Dry weight 400mg

 

MtoZ Biolabs is dedicated to offering comprehensive histone modification analysis services to research institutions and pharmaceutical companies worldwide, driving forward the depth of biomedical research. For more details or to discuss potential collaborations, please contact us at your convenience.