How Can Protein Bands Be Analyzed in SDS-PAGE Purity Assessment?
Band Resolution and Integrity
The clarity and integrity of protein bands should be carefully examined. Diffuse or smeared bands may indicate protein aggregation, degradation, or the presence of impurities.
Number of Bands
The presence of multiple bands within a single lane suggests the coexistence of multiple protein species, such as contaminants or degradation products. If the sample is expected to contain a single purified protein, only one distinct band should be observed.
Background Cleanliness
A low-background signal is indicative of high protein purity. Excessive background staining or streaking may suggest the presence of residual buffer components, detergent interference, or non-specific binding.
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