How to Analyze Impurity Protein Data in BCA Assay?
The BCA protein assay is a common method for measuring protein concentration, based on two chemical reactions: protein binds to copper ions, forming a complex that reduces bicinchoninic acid (BCA), producing a purple product with absorbance proportional to protein concentration.
1. Data Analysis
Compare measured values with the standard curve to determine the protein concentration in unknown samples. Higher absorbance indicates higher protein concentration.
2. Units
BCA assay results are typically reported in mg/mL or μg/μL.
3. Data Interpretation
Using the slope and intercept of the standard curve, protein concentration in unknown samples can be calculated via linear regression.
BCA assay measures total protein concentration but does not distinguish protein types. For protein composition analysis, additional techniques like mass spectrometry may be required. Ensure accuracy and reproducibility for reliable results.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
Related Services
How to order?
