How to Determine Protein Purity Based on Electrophoresis Bands?
Gel electrophoresis, especially SDS-PAGE, is a common technique for separating and analyzing protein mixtures. Through this technique, researchers can separate proteins based on their size and assess protein purity by examining the band pattern on the gel after electrophoresis:
Density
Samples with higher purity typically show a single, thick band. If the sample contains multiple proteins, multiple bands may be observed.
Band Clarity
High-purity protein bands have clear and well-defined edges. Blurry or diffused bands may indicate the presence of impurities.
Background
High-purity proteins should have a clean background without scattered staining or blurry bands.
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