Immobilized Antibody Sequencing Service

Immobilized antibody sequencing involves attaching antibodies to a solid substrate, such as agarose beads, silica gel matrices, or glass slides, followed by detailed sequencing analysis. This method employs chemical cross-linkers, such as glutaraldehyde or thiol compounds, to securely anchor the antibodies to the substrate, ensuring that their structural integrity and functional activity are maintained during subsequent processing. Once immobilized, the antibodies can be thoroughly analyzed for their amino acid sequences using high-resolution mass spectrometry, providing comprehensive structural insights. This technique enhances the stability of antibodies, increases their specificity, and improves the accuracy of sequencing.

 

MtoZ Biolabs has developed an efficient, fast, and accurate immobilized antibody sequencing platform based on high-throughput mass spectrometry (MS) technology. Our team of experienced scientists and technical experts has deep expertise in immobilized antibody and its sequencing research. We are committed to providing high-quality, efficient services to advance immobilized antibody sequencing research and its immunological applications. Free project evaluation, welcome to learn more details! Our technical specialists are available to provide a free business assessment.

 

Analysis Workflow

1. Immobilization Process

Antibodies are immobilized onto a solid support using chemical cross-linkers, ensuring their activity and specificity are preserved throughout the immobilization process.

 

2. Enzymatic Digestion

The immobilized antibodies are digested with specific proteases, such as trypsin, to produce smaller peptide fragments for detailed analysis.

 

3. Mass Spectrometry Analysis

High-Resolution mass spectrometry (HRMS) and tandem mass spectrometry (MS/MS) are employed to analyze the peptide fragments, providing critical mass-to-charge ratio (m/z) data necessary for sequencing.

 

4. Data Interpretation

Advanced bioinformatics tools are used to interpret the mass spectrometry data, reconstructing the complete amino acid sequences of the antibodies and identifying the sequences of their complementarity-determining regions (CDRs) and constant regions (CRs).

 

Service Advantages

1. High Accuracy

Utilizing advanced mass spectrometry techniques, we ensure high-resolution and precise analysis of antibody amino acid sequences.

 

2. High Throughput

Our platform can process and analyze multiple samples concurrently, significantly enhancing research efficiency and throughput.

 

3. Comprehensive Coverage

We provide exhaustive details on the full-length amino acid sequences of antibodies, including critical functional regions such as complementarity-determining regions (CDRs) and constant regions (CRs).

 

4. Customized Service

We offer bespoke sequencing solutions tailored to the specific requirements of our clients, accompanied by comprehensive technical support to ensure optimal outcomes.

 

Application

1. Optimization of Immunological Diagnostics Methods

 



 

Vashist, SK. et al. Sci Rep. 2014.

 

2. Enhancing the Performance of Immunosensors

 

 

 Gao, S. et al. Anal Chim Acta. 2022.

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Immobilized Antibody Sequencing

4. Mass Spectrometry Images

5. Raw Data