Intact Mass Analysis of Antibodies Service
In the rapidly advancing field of biologics, the accurate characterization of therapeutic antibodies is crucial for ensuring their efficacy, stability, and safety. MtoZ Biolabs’ Intact Mass Analysis of Antibodies Service leverages cutting-edge high-resolution mass spectrometry to deliver precise molecular weight measurements of intact antibody structures. By using advanced techniques such as native mass spectrometry, our service provides unparalleled insight into the composition of monoclonal antibodies, bispecific antibodies, and antibody-drug conjugates (ADCs), without the need for complex sample digestion or modifications.
Antibodies play a pivotal role in modern biomedical research and therapeutic applications. As essential components of the immune system, they are utilized in a wide array of clinical treatments, diagnostic tools, and laboratory research. Their ability to specifically bind to antigens has made them invaluable in targeted therapies, such as monoclonal antibody drugs, and in diagnostic assays like enzyme-linked immunosorbent assays (ELISA). Additionally, antibodies are indispensable in the study of disease mechanisms, biomarker identification, and vaccine development. Given their critical roles, the structural integrity of antibodies must be thoroughly assessed to ensure they perform as expected. Antibody molecules are complex, consisting of heavy and light chains held together by disulfide bonds, with various post-translational modifications (PTMs), such as glycosylation, influencing their stability and functionality. The molecular weight (MW) of an intact antibody is a key characteristic that reflects its conformation, modifications, and overall integrity. Therefore, accurate molecular weight determination is essential for verifying antibody purity, assessing heterogeneity, and ensuring consistency across batches in both research and clinical settings.
Services at MtoZ Biolabs
MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. MtoZ Biolabs introduces its Intact Mass Analysis of Antibodies service. This advanced service leverages high-resolution mass spectrometry to provide precise, quantitative measurements of the intact antibody mass, allowing for the identification of any structural variations or modifications that could impact function. By utilizing cutting-edge instrumentation, including the Thermo Fisher Orbitrap Fusion Lumos™ mass spectrometer, coupled with high-sensitivity nano-LC, MtoZ Biolabs delivers a comprehensive and accurate analysis of antibody molecular weight, structure, and post-translational modifications. This service is designed to support antibody researchers, biopharmaceutical companies, and diagnostics developers in ensuring the quality and consistency of their antibody products.
Our service utilizes a Top-Down analysis strategy, which is ideal for analyzing large, complex molecules like antibodies in their native state. Unlike Bottom-Up or Middle-Down methods, which involve digesting proteins into smaller peptides, the Top-Down approach directly measures the intact molecular mass of the antibody, preserving its full structure and post-translational modifications (PTMs) such as glycosylation and oxidation. This method offers high sensitivity and resolution, allowing for accurate detection of antibody heterogeneity, isoforms, and structural variations. By analyzing the intact antibody, we can assess its molecular weight, modifications, and potential aggregation, providing comprehensive insights into its quality and functionality. The Top-Down strategy is particularly suited for antibodies, ensuring precise characterization and reliable data for quality control or research applications.
Analysis Workflow
Figure 1. Main Steps Required for Top-Down Protein MS Experiment
Service Advantages
1. Advance Analysis Platform: MtoZ Biolabs established an advanced Intact Mass Analysis of Antibodies Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all intact mass analysis data of antibodies providing clients with a comprehensive data report.
4. Fast Turnaround: The process from sample handling to report generation is efficient, shortening the analysis cycle.
Case Study
Quantification of Antibody-Drug Conjugates (ADC) at the Intact Protein Level in Biological Samples
Barbero, L. M. et al. Eur J Pharm Sci. 2023.
Figure 2. MS Spectra of SST (ADC:mAb 1:1 mix at 10.0ng/mL) and ADC Spiked Plasma Samples (5 μL Injected)
Sample Submission Suggestions
1. Please provide purified samples whenever possible.
2. Ensure that the samples are free from any contamination by impurities.
*Note: For detailed information on sample submission, please do not hesitate to contact us.
FAQ
Q: Do you offer analysis for different types of antibodies, including Antibody-Drug Conjugates (ADCs) or bispecific antibodies?
Yes, our Intact Mass Analysis of Antibodies service is capable of analyzing a wide range of antibody types, including monoclonal antibodies (mAbs), bispecific antibodies, multispecific antibodies, Antibody-Drug Conjugates (ADCs), and more. Whether you are working with standard antibodies or more complex antibody formats, our service provides detailed insights into their intact mass, structural modifications, and heterogeneity. We use advanced mass spectrometry techniques to accurately assess molecular weight, conjugation status, and any post-translational modifications, ensuring high-quality data for all types of antibodies.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Relevant Mass Spectrometry Parameters
4. Raw Data Files
5. Detailed Information Report on Intact Mass Analysis of Antibodies
How to order?
