Intact Mass Analysis Service

Intact Mass Analysis Service is a high-resolution mass spectrometry technique used to determine the total molecular weight of biological macromolecules, such as proteins, antibodies, and viral particles. This service requires no sample degradation and enables the rapid, accurate acquisition of complete mass information, allowing for the detection of post-translational modifications, variants, and impurities. It ensures structural integrity and purity, which is essential for product consistency. In biopharmaceuticals and proteomics research, intact mass analysis plays a critical role in quality control, validating the stability and consistency of different product batches, and providing a reliable basis for scientific research and drug development, thus enhancing research efficiency and reducing production risks.

 

Service at MtoZ Biolabs

MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. We have established a powerful and professional intact mass analysis platform, which includes Thermo Fisher Q Exactive HF and Obitrap Fusion Lumos mass analyzer system, coupled with Nano-LC system. Our intact mass analysis service consists of ESI-Q TOF-MS Intact Mass Analysis, MALDI-TOF-MS Intact Mass Analysis, Protein Molecular Weight Determination, etc. If you are interested in our service or have other analysis requirements, please contact us. We will do our utmost to meet your needs.

 

Analysis Workflow

 



 

Service Advantages

1. High-resolution Detection for Precise Molecular Characterization: Leveraging advanced mass spectrometry equipment and optimized workflows, MtoZ Biolabs provides exceptionally high-resolution molecular mass analysis, allowing for the fine detection of subtle structural differences in proteins. This enables clients to accurately understand critical molecular characteristics of their samples, supporting high-standard research and production.

 

2. Specialized Sample Handling and Analytical Strategies: With extensive expertise in mass spectrometry and proteomics, MtoZ Biolabs designs optimized analytical workflows tailored to different sample types, minimizing errors and enhancing data quality. This ensures clients receive accurate, reliable mass analysis results, forming a solid foundation for downstream applications.

 

3. Accurate Handling of PEGylated Protein Heterogeneity: For complex PEGylated proteins, MtoZ Biolabs utilizes MALDI-MS and other specialized techniques to obtain overall molecular weight, avoiding the interference of complex signals often encountered with LC/MS, and providing clients with clear and accurate analytical results.

 

4. Comprehensive Variant and Mutation Detection: MtoZ Biolabs offers specialized peptide mapping strategies to identify low-abundance amino acid misincorporations, point mutations, and frameshifts, ensuring that products meet the highest quality standards.

 

Sample Submission Suggestions

1. We accept various types of protein sample, including monoclonal antibodies, insulin, hemoglobin, enzymes, immunoglobulins, therapeutic monoclonal antibodies. If you have other type of protein to be analyzed, please contact us.

 

2. Sample Volume: The volume and amount of the sample should be adjusted according to the specific measurement methods and instrument requirements. It is generally recommended to provide sufficient sample volume to allow for multiple measurements and validations, ensuring data reliability.

 

3. Sample Preservation: Avoid repeated freeze-thaw cycles to prevent protein degradation or loss of phosphorylation modifications. It is recommended to thaw the sample once before measurement and maintain it at an appropriate temperature during processing.

 

Case Study

1. Integrating Intact Mass Analysis and Middle-Down Mass Spectrometry Approaches to Effectively Characterize Trastuzumab and Adalimumab Structural Heterogeneity

 



Zhu, W. et al. J Proteome Res. 2021. 

 

2. Intact Mass Analysis of Monoclonal Antibodies by Capillary Electrophoresis—Mass Spectrometry

 



Han, M. et al. J Chromatogr B Analyt Technol Biomed Life Sci. 2016. 

 

FAQ

1. How to control sample quantity and concentration to ensure detection sensitivity when the sample is limited?

When sample quantity is limited, it’s recommended to optimize injection volume and use high-sensitivity mass spectrometry instruments while minimizing sample loss to ensure signal intensity. Adjusting sample concentration based on analysis objectives, extending detection time, or increasing accumulation cycles can also enhance sensitivity. Utilizing micro-injection devices and low-background interference reagents can further support obtaining reliable data, even with limited sample amounts.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Intact Mass Analysis 

5. Mass Spectrometry Image

6. Raw Data