Intact Protein Mass Spectrometry Service

Intact Protein Mass Spectrometry Service uses highly sensitive mass spectrometry technology to analyze the molecular mass, composition, post-translational modifications, and higher-order structural features of intact proteins, maintaining their native and unaltered state. Intact protein mass spectrometry (IP-MS) plays a crucial role in understanding protein integrity, identifying sequence variations, and characterizing modifications that may impact protein function or stability. In biopharmaceutical development, where protein purity and quality are critical, intact protein mass spectrometry service offers significant advantages; in basic research, it aids in understanding protein conformations and interactions. By providing precise and comprehensive analysis of proteins, intact protein mass spectrometry service supports accurate biomarker discovery, therapeutic protein development, and more efficient drug design, enhancing our understanding of complex biological systems and driving innovation in both research and industry.

 

Service at MtoZ Biolabs

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. We have established a powerful and professional intact protein analysis platform, which includes Thermo Fisher Q Exactive HF and Obitrap Fusion Lumos mass analyzer system, coupled with Nano-LC system. Our intact protein mass spectrometry service include intact protein molecular weight determination, polymer-protein complex molecular weight determination, protein purity and integrity assessment, protein degradation and stability analysis, and peptide mapping and sequence analysis, among others. With an experienced team and advanced equipment, MtoZ Biolabs provides high-quality, reliable analytical results to support scientific discovery and innovative research. If you are interested in our service, please feel free to contact us.

 

Analysis Workflow

 



 

Service Advantages

1. High Accuracy and High Resolution: Using advanced mass spectrometry techniques (such as MALDI-TOF or ESI-MS), we provide precise protein molecular weight determination, ensuring accurate identification of proteins and their subtypes.

 

2. Fast and Efficient: With an efficient analytical workflow, intact protein mass spectrometry service offers high-quality molecular weight data in a short amount of time.

 

3. Applicable to a Wide Range of Samples: Whether for purified proteins or complex samples, our intact protein mass spectrometry service can provide precise molecular weight determination, meeting the needs of various research fields.

 

4. High Sensitivity: Even low-abundance protein samples can be accurately detected, intact protein mass spectrometry service in MtoZ Biolabs caters to a wide range of research needs from basic science to clinical studies.

 

5. Modification Analysis: In addition to routine molecular weight determination, intact protein mass spectrometry service can also detect the impact of post-translational modifications (such as phosphorylation, glycosylation, etc.) on protein molecular weight, aiding in the analysis of complex protein structures.

 

Sample Submission Suggestions

1. Sample Purity: The sample should be purified as much as possible to minimize impurities that could interfere with mass spectrometry analysis. Purity is generally required to be above 80%.

 

2. Sample Concentration: It is recommended to provide protein samples at a concentration typically between 1-10 μM, with specific requirements adjustable based on sample type and analysis method.

 

3. Solvent Requirements: Samples should be dissolved in a buffer suitable for mass spectrometry analysis, such as deionized water, PBS, or other appropriate salt-free buffers. Avoid using solvents containing organic compounds or solutions with excessively high salt concentrations.

 

4. Sample Storage: If the sample cannot be analyzed immediately, it should be stored at -80°C or -20°C and kept from repeated freeze-thaw cycles to maintain protein stability.

 

5. Special Requirements: For post-translationally modified proteins or complexes, additional information such as the type of modification or complex composition may be required to ensure optimal analysis results.

 

Case Study

1. Native Ambient Mass Spectrometry of an Intact Membrane Protein Assembly and Soluble Protein Assemblies Directly from Lens Tissue

 

 

Hale, OJ. et al. Angew Chem Int Ed Engl. 2022.

 

2. Intact mAb LC–MS for Drug Concentration from Pre-Clinical Studies: Bioanalytical Method Performance and In-Life Samples

 

 

Kellie, JF. et al. Bioanalysis. 2020. 

 

Applications

1. Intact Protein Mass Spectrometry Service Used For Drug Discovery

 

 

Pu, F. et al. Anal Chem. 2022. 

 

FAQ

Q1: How to Prepare Samples for Intact Protein and Peptide Mass Spectrometry Analysis:

Answer: 1. Proteins must be pure, meaning no other proteins should be present.

2. Poteins must be lyophilized (freeze-dried) from a low-salt buffer. Please specify the volume of the liquid before drying.

3. The protein concentration before drying must be at least 0.1 mg/mL.

4. The solution must be a low-salt buffer, such as 50 mM ammonium bicarbonate. For TRIS or phosphate buffers, the maximum salt concentration should not exceed 20 mM. NaCl and surfactants (e.g., SDS) must not be present.

 

Intact protein mass spectrometry service provides precise guidelines for sample preparation, ensuring purity, buffer composition, and optimal conditions to achieve accurate and reliable mass spectrometry results.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Intact Protein Mass Spectrometry Analysis 

5. Mass Spectrometry Image

6. Raw Data