Mass Spec Protein Identification Service

Mass spec protein identification utilizes mass spectrometry to accurately identify and quantify proteins in biological samples based on their mass-to-charge (m/z) ratio. Mass spectrometry (LC-MS/MS or MALDI-TOF MS) analyzes complex protein mixtures, delivering high-sensitivity and high-accuracy protein spectra. It not only accelerates the advancement of proteomics research but also provides scientists with reliable data support, driving progress in biomedical and life sciences. Mass spec protein identification include sample preparation, protein digestion, mass spectrometry analysis, data processing, and protein identification, making it applicable to various fields such as basic research, disease biomarker discovery, drug development, and biotechnology applications.

 

Service at MtoZ Biolabs

MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. MtoZ Biolabs offers professional mass spec protein identification service characterized by advanced equipment and instruments, which includes Thermo Fisher Q Exactive HF and Obitrap Fusion Lumos mass analyzer system, coupled with Nano-LC system. In the fields of proteomics, metabolomics, and small molecule compound identification, we have accumulated a wealth of successful cases. We offer high-quality and reliable mass spec protein identification solutions to meet the diverse needs of both research and industry. If you are interested in our services, please feel free to contact us—we are dedicated to serving you.

 

Analysis Workflow

 



Wang, Z. et al. ACS Meas Sci Au. 2024.

 

Service Advantages

MtoZ Biolabs' mass spec protein identification service features the following four key advantages:

1. Precise Identification of Multiple Isoforms: MtoZ Biolabs utilizes advanced bioinformatics tools and comprehensive databases to accurately identify various protein isoforms generated through alternative splicing. This ensures comprehensive coverage and precise identification of complex proteomes.

 

2. Efficient Detection of Post-Translational Modifications: Mass spec protein identification in MtoZ Biolabs is equipped with specialized enrichment techniques and customized mass spectrometry methods, enabling effective detection and identification of common post-translational modifications such as phosphorylation, glycosylation, and acetylation. This provides precise analysis of functionally diverse proteins.

 

3. Analysis of Complex Protein Complexes: By leveraging cross-linking, affinity purification, and advanced separation technologies, MtoZ Biolabs can isolate and identify proteins within multi-molecular complexes. This allows for in-depth analysis of intricate protein interaction networks, catering to diverse research needs.

 

4. Personalized Proteomics Solutions: By integrating genetic variation databases and personalized analysis methods, MtoZ Biolabs takes into account allele variants and single-nucleotide polymorphisms (SNPs) during the protein identification process. This enhances the accuracy and comprehensiveness of identification results, meeting the research needs of different individuals.

 

Case Study

1. Mass Spectrometry and Proteome Analysis to Identify SARS-CoV-2 Protein from COVID-19 Patient Swab Samples



Banerjee, A. et al. STAR Protoc. 2022.

 

2. Deep Learning Powers Protein Identification from Precursor MS Information

 



Dai, Y. et al. J Proteome Res. 2024.

 

3. Identification of Novel Interferon  Responsive Protein Partners  of Human Leukocyte Antigen  A (HLA‐A) Using Cross‐Linking Mass  Spectrometry (CLMS) Approach

 



Singh, A. et al. Sci Rep. 2022.

 

Applications

1. Mass spec Protein Identification in Research of Disease Biomarker and Signaling Pathway

 



Chang, L. et al. Sci Rep. 2017.

 

FAQ

1. How can low-abundance proteins in complex protein mixtures be effectively separated and identified to avoid the masking effect of high-abundance proteins?

To effectively separate and identify low-abundance proteins in complex protein mixtures and avoid the masking effect of high-abundance proteins, the following methods can be employed. First, use affinity columns to remove high-abundance proteins such as albumin and immunoglobulins, thereby reducing interference. Second, apply multi-level separation techniques, such as high-performance liquid chromatography (LC) or two-dimensional gel electrophoresis (2-DE), to further separate the proteins. Then, utilize specialized enrichment methods, such as immunoprecipitation or metal oxide affinity chromatography (MOAC), to concentrate specific low-abundance proteins. Finally, combine these steps with high-sensitivity mass spectrometry analysis to ensure the detection and identification of low-abundance proteins.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Protein Identification 

5. Mass Spectrometry Image

6. Raw Data