Methylation Protein Analysis Service
Methylated proteins undergo modifications where methyl groups (-CH3) are added to specific amino acid residues, such as lysine and arginine. This process is catalyzed by methyltransferases, which transfer methyl groups from S-adenosylmethionine (SAM) to the amino acid residues. Methylation can result in mono-methylation (one methyl group), di-methylation (two methyl groups), or tri-methylation (three methyl groups).
Methylation modifications are crucial for gene expression regulation, cell differentiation, signal transduction, and chromatin remodeling. Analyzing methylated proteins helps to: (1) Understanding mechanisms of gene expression regulation; (2) Exploring cell functions and signaling pathways; (3) Discovering disease biomarkers; (4) Developing novel therapeutic strategies.
MtoZ Biolabs offers methylation Protein Analysis Service, delivering high-quality data, offering deep insights into methylation roles in biological processes to advancing research and clinical applications. Free project evaluation, welcome to learn more details! Our technical specialists are available to provide a free business assessment.
Analysis Workflow
1. Sample Preparation
Clients provide cell or tissue samples, and we perform protein extraction and digestion.
2. Peptide enrichment
Methylated peptides are selectively captured using specific antibodies or other enrichment techniques.
3. Liquid Chromatography Separation
The enriched peptides are separated via liquid chromatography (LC) to reduce sample complexity.
4. Mass Spectrometry Analysis
Conducting high-sensitivity, high-resolution peptide detection and identification using advanced mass spectrometers.
5. Data Analysis and Reporting
Using professional data analysis software to identify methylation sites and provide detailed analysis reports.
Service Advantages
1. High Sensitivity and High Resolution
Our service can accurately determine the mass-to-charge ratio (m/z) and the modification sites of peptides, enabling the detection of even low-abundance methylation modifications.
2. Precise Localization of Modification Sites
We can identify the exact location of methylation modifications (such as lysine or arginine residues), providing detailed structural information.
3. Wide Applicability
Our platform can analyze various types and complexities of biological samples, including cells, tissues, and body fluids, making it suitable for diverse biomedical research and clinical applications.
Sample Submission Requirements
For more sample details, please consult our technical team.
Applications
1. Disease Research
Identifying disease-related methylation markers to reveal disease mechanisms.
2. Drug Development
Screening potential drug targets and evaluating the impact of drugs on protein methylation.
3. Biomarker Discovery
Finding methylation markers related to specific biological processes or disease states.
4. Gene Regulation Research
Studying the role of methylation modifications in gene expression regulation.
Deliverables
1. Experimental Procedures
2. Relevant Mass Spectrometry Parameters
3. Detailed Information on Methylation Protein Analysis
4. Mass Spectrometry Images
5. Raw Data
How to order?
