Multiomics Analysis FAQ

• • What Are the Differences and Application Ranges of 16S, 18S, and ITS in Amplicon Sequencing

  16S and 18S refer to the small subunit ribosomal RNA genes in bacteria and eukaryotes, respectively. These genes are involved in the process of protein synthesis within the cell. ITS refers to the Internal Transcribed Spacer, a non-coding region located between the coding regions in the ribosomal RNA genes of fungi and other eukaryotes.

• • Should OTU or ASV Clustering Be Used in 16S Sequencing?

  In 16S rRNA sequencing, two main clustering approaches are commonly employed: OTU (Operational Taxonomic Units) clustering and ASV (Amplicon Sequence Variants) clustering. Each method offers distinct advantages and limitations.   OTU Clustering 1. Advantages OTU clustering is a conventional approach that groups sequences into OTUs based on a predefined similarity threshold, typically set at 97%. This method effectively mitigates the impact of random sequencing errors and has been extensively applied i......