What Are the Sample Submission Requirements for Regular Transcriptome Sequencing
Sample Submission Requirements for Standard Transcriptome Sequencing:
1. Sample Type
The samples should consist of either total RNA or enriched mRNA.
2. Quantity and Quality
A minimum quantity of 1-5 µg of RNA is required. The RNA should be of high quality, with an RNA Integrity Number (RIN) of 7.0 or higher, as assessed by standard integrity evaluation methods.
3. Purity
The purity of the RNA should be confirmed by an OD260/280 ratio between 1.8 and 2.0, indicating minimal contamination by proteins or other organic substances.
4. Concentration
Accurate measurement of RNA concentration should be performed using devices such as Qubit or NanoDrop, ensuring precise quantification.
5. Packaging and Transportation
Samples must be transported frozen, under appropriate conditions using dry ice or liquid nitrogen, to maintain sample integrity.
Prior to sample submission, it is recommended to engage in dialogue with laboratory personnel to ascertain specific procedural requirements and gain insights to facilitate seamless experimental workflow.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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