N-Glycosylation Site Analysis Service

N-glycosylation is a common form of glycosylation modification in eukaryotes. Under the catalytic action of glycosyltransferases, oligosaccharide chains form amide bonds with the asparagine residues of proteins, becoming covalently attached. N-glycosylation sites refer to the amino acid residues where N-glycosylation modifications occur. The analysis of N-glycosylation sites identifies and localizes specific amino acid residues that undergo N-glycosylation in proteins through proteolysis and mass spectrometry techniques. The analysis results reveal the structure and function of proteins, which is crucial to ensuring the optimal performance of biological products. In-depth analysis of N-glycosylation sites may unveil more biological insights and guide the development of novel therapeutics.

 

MtoZ Biolabs has developed a series of reliable and high-performance glycosylation characterization methods based on liquid chromatography-high resolution mass spectrometry (LC-MS/MS). We offer comprehensive one-stop N-glycosylation site analysis services, including N-glycan analysis, N-glycosylation site identification, and detailed glycopeptide analysis workflows. Please feel free to reach out to us for further information.

 

Analysis Workflow

N-glycosidase (PNGase) is used in ¹⁸O-labeled water (H₂¹⁸O) to enzymatically cleave N-glycans from asparagine (Asn) residues, converting them into ¹⁸O-labeled aspartic acid, which introduces a mass shift of 2.99 Da at the modification site. Mass spectrometric analysis can precisely detect this mass shift of 2.99 Da, enabling the accurate identification and characterization of N-glycosylated peptides and their modification sites.

 



 

Experimental Instruments

1. NanoLC: Vanquish NEO

2. MS: Orbitrap Fusion Lumos

 

Sample Submission Requirements

 



For more sample details, please consult our technical team.

 

Applications

• Intercellular Communication Research
• Infection and Immunity Research
• Tumorigenesis Research
• Disease Biomarker Research

 

Deliverables

In the technical report, MtoZ Biolabs will provide you with detailed technical information, including:

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. N-Glycosylation Site Analysis Details

4. Mass Spectrometry Images

5. Raw Data