N-Glycosylation Site Profiling Service

Protein N-glycosylation is a vital post-translational modification in life sciences, playing a significant role in both physiological and pathological processes. Precise analysis of N-glycosylation sites—including evaluating both the overall occupancy of these sites and the specific sugar structures at each site—is critical for understanding the biosynthesis and functionality of glycoproteins.

 

In cellular biology, the occupancy rate of N-glycosylation sites reflects the level of protein glycosylation, revealing various biochemical parameters in the endoplasmic reticulum such as substrate concentrations and enzyme activities. The functionality and stability of glycoproteins are largely dependent on these glycosylation patterns, making accurate quantification essential for insights into protein functions and health-related studies.

 



Mastrangeli, R. et al. Molecules. 2023.

 Figure1. Glycosylation Site Analysis Workflow

 

Services at MtoZ Biolabs

MtoZ Biolabs utilizes state-of-the-art liquid chromatography-mass spectrometry (LC-MS) for the quantitative analysis of N-glycosylation sites, ensuring high precision and accuracy. We offer a range of methods, both labeled and unlabeled, to accommodate diverse research needs. Techniques such as SILAC, TMT, dimethylation, and iTRAQ enable the simultaneous processing and analysis of multiple samples in a single experiment.

 

1. Labeled Quantitative Analysis of N-Glycosylation Sites

• Efficient and precise analyses using methods like ¹⁸O labeling catalyzed by proteases, SILAC, and iTRAQ.
• Identification of N-glycosylation sites is facilitated by treating proteins with PNGaseF and employing the ¹⁸O labeling method.
• Glycopeptides are isolated and analyzed in detail using C18-LC-MS/MS or MALDI-TOF-MS/MS

 

2. Unlabeled Quantitative Analysis of N-Glycosylation Sites

• Quantification is achieved by directly measuring the intensity or area of peptide ions, streamlining the experimental workflow.
• Specific cleavage of proteins by PNGase F, followed by enrichment of N-glycopeptides through HILIC and determination of site occupancy via LC-ESI-MS/MS.

 

Service Advantages

1. A variety of technological platforms and labeling options.
2. Rigorous quality control ensures the reliability and accuracy of our results.
3. Rapid processing times and a comprehensive one-stop service experience.
4. Detailed reports including experimental procedures, equipment settings, raw data, glycosylation analysis, and extensive bioinformatics insights.

 

Sample Submission Requirements

 



For more sample details, please consult our technical team.

 

Applications

• Intercellular Communication Research
• Infection and Immunity Research
• Tumorigenesis Research
• Disease Biomarker Research

 

MtoZ Biolabs, with its vast experience and technical proficiency, offers customized services tailored to meet individual client needs. Our efficient and precise services help clients save time and costs. For more information or consultations, please contact us.