Peptide De Novo Sequencing Service
Peptide de novo sequencing is a method of peptide sequence analysis that does not depend on existing databases. It is primarily used to determine the amino acid sequences of unknown or newly discovered peptides. This technique plays a crucial role in biomedical research and applications, providing high-resolution peptide sequence information. It is especially valuable when known sequences are unavailable in existing databases, aiding researchers in understanding complex biological processes and mechanisms, and fostering the development of new drugs and therapies.
MtoZ Biolabs has developed a highly efficient, rapid, and accurate peptide de novo sequencing platform leveraging high-throughput mass spectrometry (MS) technology. Our team comprises experienced scientists and technical experts with extensive expertise in peptide and protein sequencing. We are dedicated to delivering high-quality and efficient services to advance peptide de novo sequencing. Free project evaluation，Welcome to learn more details! Our technical specialists are available to provide a free business assessment.
Chong, KF. et al. J Bioinform Comput Biol. 2012.
Analysis Workflow
1. Sample Preparation
Extracting and purifying the target peptide.
2. Mass Spectrometry Analysis
(1) MS1: Measuring the overall molecular weight of the peptide.
(2) MS2: Utilizing CID or other techniques to fragment the peptide further and measuring the mass of the resulting ions.
3. Data Acquisition and Spectrum Analysis
(1) Employing software to convert mass spectrometry data into fragment ion spectra.
(2) Analyzing the mass differences between b ions and y ions to deduce the peptide's amino acid sequence.
4. Result Validation
(1) Comparing with known sequence databases to verify sequence accuracy.
(2) Confirming the sequence with additional experimental methods, such as Edman degradation.
Service Advantages
1. High Accuracy and Efficiency: Our combination of HCD and ETD techniques ensures high-resolution and high-quality peptide de novo sequencing, providing fast and accurate results.
2. Comprehensive Analysis: By utilizing multiple protease digestion techniques, we ensure the complete identification of target peptide sequences.
3. Experienced Team: Our professional team of scientists provides accurate data analysis and interpretation.
4. Innovative Technology: We leverage advanced mass spectrometry technology and data processing algorithms to significantly enhance sequencing accuracy and efficiency.
Applications
1. Discovery of New Peptides and Proteins: Identifying unknown peptides or proteins, particularly in new species or newly discovered biological samples.
2. Antibody Sequencing: Determining the amino acid sequences of antibody light and heavy chains, which is crucial for developing antibody drugs.
3. Post-Translational Modifications (PTMs) Research: Analyzing post-translational modifications such as phosphorylation and glycosylation, aiding in understanding protein function and regulatory mechanisms.
4. Biomarker Discovery: Identifying new biomarkers in disease research, promoting the development of diagnostics and therapies.
Deliverables
1. Experimental Procedures
2. Relevant Mass Spectrometry Parameters
3. Detailed Information on Peptide Sequencing
4. Mass Spectrometry Images
5. Raw Data
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