Peptide Purity Testing Service

The Peptide Purity Testing Service involves a comprehensive assessment of peptide samples to identify and quantify the main components and impurities. This testing is essential for ensuring peptide quality and consistency, especially in pharmaceutical, research, and diagnostic applications. By performing purity testing, one can determine whether the peptide meets expected quality standards, thus ensuring the reliability of experimental results and minimizing potential interference from impurities. Additionally, accurate purity data aids in optimizing synthesis processes, enhancing the safety and efficacy of products. High-purity peptides improve therapeutic effectiveness and reduce the risk of side effects, making peptide purity testing an indispensable step in both research and commercial applications.

 

Service at MtoZ Biolabs

MtoZ Biolabs’ peptide purity testing service uses precise reversed-phase high-performance liquid chromatography (RP-HPLC) combined with mass spectrometry (MS) to ensure accurate determination of peptide purity and molecular weight, meeting the stringent requirements of various research and application needs. We offer different purity level testing options, ranging from >98%, 95%-98%, >85%, to 70%, covering a wide range of applications. Through strict quality control standards, we ensure peptide purity and consistency throughout the entire analysis process, providing reliable data support. This enables clients to select the most suitable peptide purity for specific experimental needs, optimizing research outcomes and commercial applications.

 

Analysis Workflow

 



H, Lee. et al. Bull Korean Chem Soc. 2022.

 

Service Advantages

1. High Accuracy and Reliability: Advanced RP-HPLC and mass spectrometry techniques are used to ensure high accuracy and consistency in determining peptide sample purity and molecular weight.

 

2. Multiple Purity Level Options: Our peptide purity testing service accepts a variety of purity testing options, including >98%, 95%-98%, >85%, and 70%, to meet client needs across different application scenarios.

 

3. Strict Quality Control: The entire testing process adheres to rigorous quality control standards to ensure the accuracy and reliability of results.

 

4. Wide Application Compatibility: The service supports a broad range of research and commercial applications, including crystallography, drug development, ELISA testing, and antibody production, helping clients optimize the use of peptide products.

 

Sample Submission Suggestions

1. Sample Type and Condition: Submitted samples should be pure peptide products, ensuring they are free from contamination or degradation. Liquid samples must be well-sealed, and solid samples should be securely packaged to prevent moisture.

 

2. Sample Quantity: Each analysis project typically requires an adequate amount of sample, with the specific quantity depending on the analysis method and project requirements. It is recommended to provide sample amounts ranging from 1 mg to multigram quantities to ensure the accuracy and reproducibility of the analysis.

 

3. Sample Labeling and Information: Each sample must be clearly labeled, including the sample name, batch number, supplier information, and submission date. Additionally, detailed sample descriptions should be provided, such as purity, molecular weight, and any known modifications.

 

4. Storage and Transportation Conditions: Samples should be stored according to the recommended conditions, such as low temperatures (−20 °C or −80 °C) to prevent degradation. During transportation, appropriate cold chain packaging should be used to ensure that samples remain stable throughout the shipping process.

 

5. Special Requirements: If samples have special properties or require specific analysis methods, please inform MtoZ Biolabs in advance to arrange the appropriate analysis plan and equipment.

 

If you have any questions or need further guidance, please feel free to contact us.

 

Case Study

1. Identification and Accurate Quantification of Structurally Related Peptide Impurities in Synthetic Human C-Peptide by Liquid Chromatography–High Resolution Mass Spectrometry

 



Li, M. et al. Anal Bioanal Chem. 2018.

FAQ

1. How to address solubility issues during sample preparation?  

For peptides with strong hydrophobicity, various solvent combinations can be tried to increase solubility. For example, a small amount of organic solvents such as acetonitrile or DMSO can be gradually added to the buffer solution to allow the peptide molecules to dissolve fully. Additionally, co-solvents or surfactants can be incorporated during preparation, but it is essential to ensure that these additives do not interfere with subsequent analyses.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Peptide Purity Testing 

5. Mass Spectrometry Image

6. Raw Data