Protein Amino Acid Composition Analysis Service

Proteins are complex biological macromolecules formed by amino acids linked through peptide bonds. The specific types and sequences of amino acids dictate the protein's structure and function. The three-dimensional structure of a protein is inherently determined by its amino acid sequence, where the chemical properties of each amino acid (such as hydrophilicity, hydrophobicity, and acidity) influence protein folding and final structure, subsequently affecting its biological function. Amino acid composition analysis is a critical method for studying protein structure and function. This analysis allows for the identification and quantification of amino acids within proteins, which is vital for protein identification, especially in the characterization of unknown proteins and comparative analysis across different samples. In the production of protein therapeutics, amino acid composition analysis serves as a crucial quality control measure to ensure product consistency and purity.

MtoZ Biolabs has established a powerful and professional protein amino acid composition analysis platform, which includes Thermo Fisher Q ExactiveHF and Obitrap Fusion Lumos mass analyzer system, coupled with Nano-LC system. Our aim is to provide the most professional support for our clients’ research. Free project evaluation, welcome to learn more details! Our technical specialists are available to provide a free business assessment.

Analysis Workflow

1. Sample Processing

Pre-treatment of the samples is performed, including protein purification and quantification.

2. Protein Hydrolysis

Protein samples undergo hydrolysis in 6N hydrochloric acid at elevated temperatures, typically for 24 hours.

3. Derivatization

Hydrolyzed amino acids are derivatized to enhance detection sensitivity.

4. HPLC/CE Analysis

Derivatized amino acids are separated and detected using high-performance liquid chromatography (HPLC) or capillary electrophoresis (CE).

5. Data Analysis and Reporting

The results are analyzed, and a comprehensive report is generated, detailing amino acid composition and quantitative data.

Applications

1. Biomedical Research

Amino acid composition analysis elucidates the relationship between protein structure and function, supporting research into disease mechanisms and drug development.

2. Food Science

Evaluation of the amino acid composition in food proteins can assess nutritional value of the food.

3. Industrial Applications

Amino acid composition analysis helps optimize protein production and modification in protein engineering and biotechnology fields.

Service Advantages

1. High Precision and Sensitivity

Our advanced HPLC and CE technologies guarantees the accuracy and sensitivity of analytical results.

2. Expert Team

Our experienced technical team offers comprehensive services from sample preparation to data analysis.

3. Customized Solutions

We offer tailored analysis solutions to meet specific client needs across diverse research and application domains.

4. Rapid Turnaround

Our streamlined analytical processes ensure the prompt delivery of high-quality reports.