Protein Full Spectrum Analysis Service

Proteins, the direct executors of biological functions, shape life's diversity through their complex and varied forms. To advance our understanding of these biological building blocks, MtoZ Biolabs offers comprehensive proteomic analysis using cutting-edge mass spectrometry. This method enables the detailed identification and analysis of proteins and their modifications in biological samples like serum, urine, and cell lysates, ensuring a thorough understanding of their types, quantities, and post-translational modifications.

 

MtoZ Biolabs excels in delivering top-tier full-spectrum protein analysis services, aiding researchers in deciphering biological complexities. At its heart, the lab employs a combination of shotgun proteomics, high-performance liquid chromatography (HPLC), and mass spectrometry (MS) to identify and quantify proteins in intricate biological samples accurately.

 

Analysis Workflow

1. Sample Preparation

Biological samples are preprocessed and purified to prepare for analysis.

 

2. Protein Digestion

Proteins are enzymatically digested into manageable peptides.

 

3. Peptide Separation

Using HPLC, peptides are separated based on their physicochemical properties.

 

4. Mass Spectrometry Detection

Peptides undergo mass measurement and sequence analysis in a mass spectrometer, which provides detailed information about their mass-to-charge ratios and fragment ions.

 

5. Data Analysis

Advanced bioinformatics tools and databases are utilized to interpret mass spectrometry data, identifying protein types, quantities, and modifications.

 

6. Report Writing

A comprehensive report is produced, detailing protein identities, modification sites, quantities, and analytical interpretations.

 



Rost, J. et al. Food Res. Int. 2019.

Figure 1. A Label-Free Shotgun Proteomics Analysis Workflow

 

Service Advantages

• High Sensitivity and High Throughput: Capable of identifying thousands of proteins simultaneously, meeting the needs of large-scale proteomics research.
• High Accuracy: Combines advanced mass spectrometry technology and bioinformatics tools to ensure the accuracy and reliability of protein identification.
• Wide Applicability: Supports various types of biological samples, including cells, tissues, bodily fluids, etc.
• Deep Analysis: Capable of revealing complex information such as protein post-translational modifications (PTMs), interaction networks, etc.

 

Sample Submission Requirements

Sample Types: Total protein samples, including but not limited to cell lysates, tissue extracts, bodily fluids, urine, etc.

 

Sample Quantity: Determined based on specific experimental needs, generally recommended to be no less than 300μg to ensure the comprehensiveness of the analysis results.

 

Applications

Disease Biomarker Discovery: Identifying potential disease markers by contrasting proteomes of healthy versus diseased states, aiding early disease detection and therapy.

 

Drug Target Research: Uncovering drug mechanisms and new targets, expediting pharmaceutical development.

 

Biomarker Validation: Confirming biomarker validity and assessing expression changes across disease states for clinical application.

 

Plant and Microbial Proteomics: Studying protein dynamics in agriculture and biotechnology, crucial for crop production and

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Protein Full Spectrum

4. Mass Spectrometry Images

5. Raw Data