Protein Post-Translational Modification Analysis Service

Post-translational modifications (PTMs) of proteins are critical chemical modifications occurring after protein biosynthesis, crucial for regulating various cellular processes such as signal transduction, gene expression, and protein-protein interactions. PTMs, which include phosphorylation, acetylation, ubiquitination, and glycosylation, modify the structural, functional, and locational attributes of proteins by appending biochemical groups to specific amino acid residues. Accurately identifying and localizing PTMs are paramount for deciphering cellular biology's complexities and their implications in diagnosing and treating diseases.

 

MtoZ Biolabs employs mass spectrometry (MS) to analyze protein PTMs, utilizing its high sensitivity and precision, which are essential in proteomic research. MS is capable of detecting and quantifying trace modifications within protein samples, offering extensive details on modification types and sites, thus facilitating comprehensive PTM analysis.

 

Analysis Workflow

1. Sample Preparation

Extract proteins from biological samples and enzymatically digest them into peptide fragments.

 

2. Enrichment of Modified Peptides

Selectively enrich target modified peptides using specific methods (e.g., immunoprecipitation or affinity chromatography).

 

3. MS Analysis

Identify peptide sequences and modification sites using liquid chromatography-tandem mass spectrometry (LC-MS/MS).

 

4. Data Analysis and Reporting

Advanced bioinformatics approaches are applied to parse data, culminating in detailed assessments of modification types and locales.

 



 

Sample Submission Requirements

 



*Customers are welcome to contact us for detailed sample requirements before sending your samples.

 

Service Advantages

1. Expertise: With profound expertise in proteomic research, we deliver top-tier analytical services globally.

 

2. Precision and Reliability: Our state-of-the-art LC-MS/MS technological platform ensures that experiments are both sensitive and specific, yielding dependable results.

 

3. Prompt Service: Recognizing the exigencies of scientific endeavors, we are committed to swift and precise service and results delivery.

 

Applications

1. Disease Research

By examining the PTM patterns in proteins associated with diseases, we can uncover functional alterations under pathological conditions.

 

2. Drug Development

Investigating the PTMs on target proteins aids in refining drug design and assessing therapeutic efficacy.

 

3. Biomarker Development

We identify and validate specific protein modifications linked to diseases, facilitating early diagnosis and prognosis.

 

If you are interested in the protein post-translational modification analysis service offered by MtoZ Biolabs, please contact us. We look forward to helping you solve your problems.