Protein Stability Assay Service
- Protein Integrity Analysis: High-resolution mass spectrometry is used to measure protein molecular weight under various conditions, determining if the protein remains intact, degrades, or truncates.
- Aggregation Detection: Molecular weight analysis helps identify aggregates such as dimers and multimers that form under extreme conditions (e.g., high temperature, pH, or salt concentration) and assesses how aggregation impacts protein stability.
- Degradation Product Identification: We identify and analyze degradation fragments resulting from unfavorable conditions, shedding light on degradation mechanisms and informing strategies to improve stability.
- Post-Translational Modification (PTM) Analysis: We detect molecular weight shifts that signify PTMs, such as phosphorylation or glycosylation, which can influence protein stability and function.
- Low-complexity samples: Gel spots or bands, approximately 1-2 cm in length.
- Medium-complexity samples: Protein mixtures, including column-purified samples.
- Moderate-to-high complexity samples: Protein mixtures such as IP-derived samples, secreted proteomes, or subcellular organelles (e.g., mitochondria, endoplasmic reticulum, lysosomes).
- High-complexity samples: Whole cell lysates, tissues.
MtoZ Biolabs is dedicated to offering professional Protein Stability Assay Service. Protein stability refers to a protein’s ability to maintain its structural and functional integrity under various environmental conditions, which is essential for the development, production, and quality control of biopharmaceuticals. By accurately measuring protein molecular weight, we assess protein integrity, purity, degradation, and aggregation under different conditions, thereby providing valuable insights into its stability characteristics.
Changes in molecular weight typically indicate structural and functional alterations in proteins. Monitoring molecular weight during stability analysis helps reveal the effects of factors such as temperature, pH, and chemical environments on protein stability. Leveraging advanced mass spectrometry technology and a skilled technical team, MtoZ Biolabs offers reliable data and scientific insights to support your protein stability studies.
Services at MtoZ Biolabs
Our Protein Stability Assay Service evaluates protein stability from the perspective of molecular weight measurement, addressing key areas including:
Analysis Workflow
Why Choose MtoZ Biolabs?
1. Advance Analysis Platform: MtoZ Biolabs established an advanced protein analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. Comprehensive Stability Assessment: Our services provide an in-depth evaluation of protein stability by focusing on molecular weight, identifying potential stability concerns for future research and development.
3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all protein analysis data providing clients with a comprehensive data report.
4. Customized Service: Tailored to the specific research needs of our clients, we offer flexible experimental design and personalized data analysis to ensure the achievement of research goals to the fullest extent.
5. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Sample Submission Suggestions
For more specific sample submission requirements, please consult our technical team.
Applications
1. Biopharmaceutical Development
Assess the stability of biopharmaceutical products under varied conditions to optimize formulations and storage protocols.
2. Protein Engineering
Identify stable protein variants by monitoring molecular weight changes, improving product stability.
3. Quality Control
Monitor degradation and aggregation during production and storage to ensure consistency and safety.
4. Basic Research
Investigate protein molecular weight changes under diverse environmental conditions, providing essential data for biological research and functional studies.
FAQ
Q1: What are the main challenges in detecting protein aggregation during stability analysis, and how can they be overcome?
Detecting protein aggregation can be particularly challenging because aggregates often form under conditions such as high temperature, extreme pH, or high salt concentrations. Aggregates such as dimers, trimers, or multimers may not only alter the molecular weight but also complicate the analysis due to their heterogeneous nature. Additionally, some aggregates may not be detected using traditional techniques like SEC or SLS. At MtoZ Biolabs, we use high-resolution mass spectrometry in combination with other analytical methods to detect and accurately quantify these aggregates. By integrating mass spectrometry-based molecular weight analysis with other orthogonal methods like DLS, we can provide a comprehensive assessment of protein aggregation, ensuring that all forms of aggregation are identified and their effects on protein stability are thoroughly evaluated.
Q2: How do PTMs affect protein stability, and how can they be identified in the stability assay?
Post-translational modifications (PTMs), such as phosphorylation, glycosylation, and acetylation, play crucial roles in regulating protein function, stability, and interactions. These modifications can influence how a protein folds, interacts with other molecules, or behaves under different environmental conditions. In the context of protein stability, PTMs may either enhance or destabilize protein structures, leading to changes in its stability profile. For instance, glycosylation can increase protein solubility and prevent aggregation, whereas dephosphorylation might cause structural destabilization. Our Protein Stability Assay at MtoZ Biolabs involves detecting molecular weight shifts that correspond to the addition or removal of PTMs. By using mass spectrometry, we can precisely identify these modifications and link them to the stability characteristics of the protein, providing invaluable information for both basic research and biopharmaceutical development.
What Could be Included in the Report?
1. Comprehensive Experimental details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs also offers other advanced protein stability analysis techniques, such as differential scanning calorimetry (DSC), thermal shift assay (TSA), Fourier-transform infrared spectroscopy (FTIR), and circular dichroism (CD). These methods, combined with molecular weight analysis, offer a comprehensive evaluation of protein stability, providing deeper insights for your research and product development. Contact our technical team for more details or to customize your solutions. We are committed to providing the highest level of service.
How to order?
