Resources

Proteomics Databases



Metabolomics Databases

• • Palmitoylation Mass Spectrometry

  Palmitoylation mass spectrometry is a sophisticated analytical technique employed to detect and characterize lipid modifications in proteins, particularly palmitoylation. This modification involves the covalent attachment of palmitoyl groups to specific amino acid side chains via ester or thioester bonds, which is critical for modulating protein function, localization, and stability. Given its dynamic and reversible nature, palmitoylation plays a pivotal role in cellular signaling pathways, protein-pr......

• • 2-DE Proteomics

  2-DE proteomics is a comprehensive analytical technique that investigates the expression, structure, function, interactions, and dynamic changes of proteins within cells, tissues, or organisms. This technique integrates isoelectric focusing (IEF) and SDS-PAGE, enabling the high-resolution separation of proteins based on their isoelectric points and molecular weights. In the first dimension, IEF separates proteins by their isoelectric points (pI) within a pH gradient, concentrating them at their respec......

• • 2D-DIGE Analysis

  2D-DIGE Analysis (Two-Dimensional Difference Gel Electrophoresis) integrates traditional two-dimensional electrophoresis with fluorescent labeling technology, enabling the resolution and quantification of multiple protein samples on a single gel. Its core advantages are high resolution and sensitivity, essential for comparing protein expression profiles. Unlike conventional two-dimensional electrophoresis (2-DE), 2D-DIGE innovatively uses fluorescent dyes such as Cy2, Cy3, and Cy5 to label different p......

• • SILAC-MS

  SILAC-MS is a widely employed technique for investigating the dynamic changes and functions of intracellular proteins. This method involves the incorporation of stable isotopes of light and heavy amino acids into cell cultures, facilitating quantitative protein analysis. The fundamental principle of SILAC-MS is the replacement of natural amino acids with isotopically labeled ones, introducing distinguishable mass differences during protein synthesis. These differences are highly discernible in mass sp......

• • Single-Cell Phosphoproteomics

  Single-cell phosphoproteomics integrates single-cell analysis with phosphoproteomics, enabling high-throughput examination of protein phosphorylation modifications at the single-cell level. This approach offers new insights into intracellular signal transduction and the regulation of biological processes. Protein phosphorylation is a prevalent post-translational modification that plays a crucial role in regulating diverse cellular functions such as signal transduction, metabolic control, cell cycle pr......

• • Saliva Proteomics for Disease Diagnosis

  Saliva proteomics investigates the protein composition and functions present in saliva, primarily through the analysis of saliva protein expression profiles using advanced proteomic technologies. As a biological fluid that is easy to collect and non-invasive, saliva serves as an ideal sample source for disease diagnosis and monitoring. It contains a complex mixture of proteins, enzymes, antibodies, and microorganisms that can reflect the physiological and pathological states of the human body. Researc......

• • Antibody Sequencing Methods and Applications

  Antibody sequencing refers to the process of determining the genetic or amino acid sequence of an antibody (immunoglobulin). Antibodies are key proteins of the immune system that recognize and neutralize foreign substances such as bacteria and viruses. A comprehensive understanding of antibody sequences is crucial for developing novel vaccines, designing therapeutic antibody drugs, and gaining deeper insights into immune system mechanisms. The core of antibody sequencing lies in accurately analyzing a......

• • What Makes Quantitative Proteomics So Powerful? A Comprehensive Breakdown

  Quantitative proteomics has emerged as a rapidly advancing field within proteomics analysis. Unlike traditional proteomics, which primarily identifies protein presence, quantitative proteomics enables precise measurement of protein expression levels. This capability has made it indispensable in diverse research areas, including disease mechanism studies, drug development, and biomarker discovery. But what makes quantitative proteomics so powerful? This review explores its key advantages, methodologies......

• • Why You’re Not Getting Reliable Protein Data and How Advanced Proteomics Can Help

  In proteomics analysis, ensuring the accuracy and stability of protein data is crucial for maintaining experimental reliability and reproducibility. However, many researchers encounter data inconsistencies, including unexpected fluctuations and deviations from anticipated results. Such challenges not only undermine the reproducibility and validity of research findings but also lead to significant loss of research time and resources. The instability of protein data can arise from multiple factors spann......

• • The Secret to High-Throughput Proteomics for Cutting-Edge Research

  High-Throughput Proteomics plays a central role in modern life sciences research, enabling researchers to simultaneously analyze thousands of proteins and uncover the dynamic changes in biological systems. However, its success depends not only on cutting-edge mass spectrometry technology but also on well-designed experiments, efficient data processing workflows, and stringent quality control measures. This review discusses the critical factors influencing the success of high-throughput proteomics, pro......