Resources

Proteomics Databases



Metabolomics Databases

• • mRNA Cap Efficiency Detection

  mRNA capping efficiency detection is a process used to evaluate the successful addition of a cap structure (typically a 7-methylguanosine triphosphate (m7G) cap) at the 5' end of mRNA molecules. This cap structure is crucial for the stability of mRNA and its translation efficiency within cells. The 5' cap of mRNA plays several key biological functions, including regulating nuclear export, protecting against degradation by exonucleases, and promoting translation.

• • Dynamic Light Scattering (DLS) Analysis of Rat Cells

  Understanding the physical and chemical properties of cells is crucial in biomedical research. As widely used experimental animals, the study of rat cells provides valuable insights into the understanding and treatment of human diseases. Dynamic Light Scattering (DLS) is a widely applied technique for measuring the size distribution of nanoparticles to microparticles in solution.

• • Identification of Plasma Protein by Tandem Mass Spectrometry

  Plasma, a liquid tissue containing rich biological information, serves not only as a medium for nutrient transfer but also as a carrier for important signaling molecules and functional proteins in the body. It encompasses thousands of proteins that play crucial roles in many physiological processes and serve as important sources of disease biomarkers.

• • Flow Cytometry Multiplex Analysis of Mouse Cells

  In biomedical research, mice play a crucial role as model organisms in studying disease mechanisms and drug development. In order to gain a deeper understanding of the complex biological characteristics of mouse cells, MtoZ Biolabs utilizes flow cytometry-based multi-factor analysis technology to provide an efficient and precise method for simultaneously analyzing multiple cell biomarkers. This technology has shown great potential in various fields such as immunology, oncology, and stem cell research.

• • Determining Protein Content in E. coli via Amino Acid Analysis

  In the field of microbiology and biotechnology, Escherichia coli, as an important model organism and industrial production microbe, accurate measurement of its protein content is of great significance for basic research and industrial applications. MtoZ Biolabs provides efficient and reliable technical means for the precise determination of protein content in Escherichia coli using advanced amino acid analysis.

• • Identifying Protein Phosphorylation Sites via Mass Spectrometry

  In mass spectrometry analysis, proteins or peptide segments are ionized into charged particles and then separated through a mass spectrometer. The mass spectrum displays the relationship between the mass-to-charge ratio (m/z) of these particles and their intensity (signal size). Interpreting the mass spectrum requires understanding the fragmentation patterns of peptide segments and the mass changes caused by phosphorylation.

• • Steps for Protein Determination Using Circular Dichroism

  Circular Dichroism (CD) is a spectroscopic technique based on electromagnetic waves that can analyze the structural features of proteins by measuring the difference in absorption of left and right circularly polarized light. It is currently widely used in determining the secondary structure of proteins, and is a fast, simple, and relatively accurate method for studying the conformation of proteins in dilute solutions.

• • Bradford Protein Concentration

  Bradford method, also known as the Coomassie Brilliant Blue method, is a fast and simple method for determining protein concentration. The method is based on the binding of proteins with a dye (Coomassie Brilliant Blue G-250), and protein concentration is determined by colorimetry.

• • How Much Protein Is Needed for Mass Spectrometry Sequencing?

  The amount of protein needed for mass spectrometry determination of protein sequence depends on several factors, including the sensitivity of the mass spectrometer, the purity of the sample, the type of analysis (such as peptide mapping, protein identification or post-translational modification analysis) and the properties of the specific protein. With advances in technology, the sensitivity of mass spectrometry is increasing and the amount of sample required is decreasing.

• • Protein Sequence Homology Analysis

  Protein sequence homology analysis is an important technique in bioinformatics, used to identify the similarity and evolutionary relationship between two or more protein sequences. Homology analysis is crucial for predicting the function of unknown proteins, studying the evolutionary history of proteins, and discovering new biological markers.