Resources

Proteomics Databases



Metabolomics Databases

• • Antigen Antibody Epitope Analysis

  The interaction between antigens and antibodies is central to immune response. This specific interaction occurs between the variable region of the antibody and a specific part of the antigen, which is called an "epitope". The binding of the antibody to its corresponding epitope is highly specific.

• • Antibody Drug Activity Testing

  Antibody drugs, especially monoclonal antibodies (mAbs), have played a crucial role in the treatment of many diseases, particularly cancer and immunological disorders. To ensure the effectiveness and safety of these antibody drugs, their activity needs to be assessed.

• • Dynamic Light Scattering Particle Analyzer: Principles and Uses

  Dynamic light scattering (DLS), also known as laser light scattering, is a technique used to measure the size of small particles (such as proteins, nanoparticles, polymers, or liposomes) in solution or suspension. DLS is based on analyzing the fluctuation of scattered light intensity from particles in a solution over time. When a laser beam is shone onto the sample, the particles in the sample scatter light.

• • IR Spectroscopy for Protein Secondary Structure

  Infrared spectroscopy, especially Fourier transform infrared spectroscopy (FTIR), is a commonly used technique for studying the secondary structure of proteins. This method is based on the vibrational absorption of the peptide bond C=O (amide I) and N-H (amide II) in different protein secondary structure components, such as α-helices, β-sheets, β-turns, and random coils.

• • Detection Methods for Host Cell Residuals in Antibody Drugs

  Monoclonal antibody drugs are produced using biotechnology, typically within host cells such as mammalian cells (e.g. CHO cells) or bacterial cells (e.g. E. coli). During the production process, there is a possibility of residual host cell contaminants entering the final drug product. These contaminants may include DNA, proteins, or other small molecules, which could potentially impact the safety and/or efficacy of the drug.

• • ADA Detection: Sample Collection, Processing, and Analysis

  "Antidrug antibodies" (ADA) usually refers to antibodies produced by the immune system against certain drugs, particularly biologics. When patients receive certain biologic therapies, such as monoclonal antibody therapy, their immune system may produce these antibodies, which can affect the safety and efficacy of the drugs. ADA testing is performed to determine whether these antidrug antibodies are present in the patient's body.

• • Experimental Steps to Determine Protein Extinction Coefficient

  The extinction coefficient of a protein is usually related to its absorbance at a specific wavelength, such as 280 nm. This is mainly because certain amino acid residues in proteins, such as tryptophan, tyrosine, and cysteine, have specific absorbance characteristics at this wavelength. The passage depicts the basic steps for determining the protein extinction coefficient.

• • Overview of Methods for Testing Collagen Content

  Collagen is the main structural protein in various tissues, especially in the skin, bones, tendons, and ligaments. Determining the collagen content can help assess the progression of certain diseases or treatment efficacy, and it can also be used to evaluate the quality of cosmetics and food products.

• • Methods and Uses of Calculating Protein Extinction Coefficient

  The extinction coefficient of a protein describes its absorbance properties under a certain wavelength of light. It is an inherent property of the protein and is linearly related to its concentration and path length. The extinction coefficient is commonly used to estimate the concentration of proteins. The extinction coefficient is defined as the absorbance at a unit concentration and unit path length.

• • Circular Dichroism: Analyzing Alpha Helices' Significance

  Circular Dichroism (CD) spectroscopy is a spectral technique used to study the chirality and secondary structure of molecules, particularly biomacromolecules such as proteins and nucleic acids. The α-helix is a common secondary structure in proteins and is crucial for understanding protein function. CD spectroscopy provides a powerful tool for analyzing α-helix structures in proteins.