Resources

Proteomics Databases



Metabolomics Databases

• • Application of Shotgun Proteomics in Protein Biomarker Discovery

  Protein biomarker discovery is a crucial area in medical research, aiding in the early detection, diagnosis, and prognosis of diseases. Shotgun proteomics, a high-throughput and comprehensive approach, has revolutionized this field by enabling the identification and quantification of a vast array of proteins in complex biological samples.

• • Advantages and Disadvantages of Shotgun Proteomics in Protein Identification

  Shotgun proteomics has emerged as a powerful technique in the field of proteomics, enabling the comprehensive identification and quantification of proteins within complex biological samples. This high-throughput approach has revolutionized protein analysis, providing deep insights into the proteome. However, like any scientific method, it comes with its own set of advantages and disadvantages.

• • Workflow of Shotgun Proteomics for Protein Identification

  Shotgun proteomics has emerged as a leading approach for the comprehensive analysis of proteins in complex biological samples. By employing high-throughput mass spectrometry, this method allows for the simultaneous identification and quantification of thousands of proteins.

• • Principle of Shotgun Proteomics in Protein Identification

  Shotgun proteomics is a powerful approach in proteomics, allowing for the comprehensive analysis and identification of proteins within complex biological samples. This method has revolutionized our understanding of proteomes by enabling high-throughput and detailed protein characterization.

• • Mechanism of Gel and IP Sample Protein Identification

  Protein identification is a fundamental aspect of biochemistry and molecular biology, crucial for understanding the roles and interactions of proteins in cellular processes. Two widely used techniques for protein identification are gel electrophoresis (gel) and immunoprecipitation (IP).

• • Circular Dichroism Spectroscopy for Chiral Compounds

  Circular dichroism spectroscopy (CD spectroscopy) is a highly effective technique used to study the structure and optical activity of chiral compounds. This technique is based on the differential absorption of left- and right-circularly polarized light by chiral molecules, which is reflected in the resulting spectrum and provides information about the molecular structure.

• • Circular Dichroism: Protein Secondary Structure Determination

  Circular Dichroism Spectroscopy, also known as CD spectroscopy, is a spectroscopic technique commonly used to study the structure of biomacromolecules such as proteins, particularly their secondary structure. CD spectroscopy provides important information about the local and global conformation of proteins, which is crucial for understanding their function and stability.

• • Protein Thermal Stability: Differential Scanning Calorimetry Detection

  Differential Scanning Calorimetry (DSC) is an analytical technique used to study the thermal stability of proteins. This method can measure the heat behavior of proteins when heated, particularly the heat released or absorbed during structural changes. DSC provides important information about protein folding and stability, which is valuable in fields such as drug design, biotechnology, and disease research.

• • Analysis of Medium Components for Recombinant Protein Vaccines

  Recombinant protein vaccines are vaccines produced using genetic engineering techniques. The principle behind these vaccines is to clone one or more antigen genes of the pathogen into host cells, allowing them to express the corresponding antigen proteins. These proteins are then extracted and used as vaccines. These vaccines effectively stimulate the immune system to produce a response against specific pathogens, providing protection.

• • Infrared Spectroscopy: Unveiling Biomolecules

  Infrared spectroscopy (IR) is a powerful experimental technique commonly used in the fields of analytical chemistry and biology, especially in the study of molecular structure and function. Infrared spectroscopy provides detailed information about molecular vibrations, which are related to the formation, length, and type of specific chemical bonds in the molecule.