Resources

• • Application of 4D-DIA Quantitative Proteomics

  The development of proteomics technologies has provided powerful tools for biological research, particularly in understanding the molecular mechanisms of complex biological systems. 4D-DIA (Four-Dimensional Data-Independent Acquisition) is an emerging technique in quantitative proteomics that combines multidimensional chromatography separation with high-resolution mass spectrometry analysis. This approach enables highly accurate and high-throughput analysis of protein expression and modifications.

• • Advantages and Disadvantages of 4D-DIA Quantitative Proteomics

  In proteomics research, Data-Dependent Acquisition (DDA) and Data-Independent Acquisition (DIA) are two commonly employed mass spectrometry techniques. Recently, 4D-DIA (Four-Dimensional Data-Independent Acquisition) quantitative proteomics has garnered significant attention from the research community. This method not only leverages the strengths of traditional DIA but also introduces ion mobility as a fourth dimension, thereby enhancing the accuracy and resolution of quantitative proteomic analyses.

• • Workflow of 4D-DIA Quantitative Proteomics

  4D-DIA (Four-Dimensional Data-Independent Acquisition) is a cutting-edge technique in modern proteomics. This approach integrates liquid chromatography, mass spectrometry, and bioinformatics to conduct high-throughput, high-precision quantitative analysis of proteins in complex biological samples. By incorporating a time dimension, 4D-DIA surpasses traditional DIA methods, enabling higher resolution data acquisition and analysis.

• • Principle of 4D-DIA Quantitative Proteomics

  Proteomics systematically examines the composition, structure, and function of proteins within cells, tissues, or organisms. Quantitative proteomics is essential for uncovering protein expression changes, interactions, and functions. 4D-DIA (Four-Dimensional Data-Independent Acquisition) has recently emerged as a cutting-edge quantitative proteomics technique, renowned for its high sensitivity, coverage, and precision.

• • From Sample to Result: Comprehensive Protein MS Analysis

  Proteins are one of the most fundamental functional molecules in living organisms, participating in nearly every biological process. Understanding the composition, structure, and function of proteins is crucial to understanding the basic principles of life. Proteomics technologies, as a powerful analytical tool, can help scientists deeply study the characteristics and functions of proteins.

• • Antibody Drug PTM Analysis: Oxidation and Deamidation

  Antibody drugs are a class of drugs that treat diseases by artificially synthesizing antibodies, such as monoclonal antibodies, artificially synthesized antibody fragments, immunotoxins, and antibody-drug conjugates. Post-translational modifications (PTMs) of proteins refer to the chemical modifications that occur during or after protein translation. They play a crucial role in cellular processes such as cell differentiation, protein degradation, signal transduction, and protein-protein interactions.

• • Determination of DAR in ADC Drugs

  Antibody-Drug-Conjugate (ADC) is an effective targeted therapy for cancer, composed of a monoclonal antibody with specific targeting properties, a potent cytotoxic small molecule drug, and a chemical linker connecting the two. The key feature of ADC drugs is the combination of highly selective monoclonal antibodies with toxic drugs through chemical conjugation. Due to its significant efficacy and superior safety, ADC is widely used in the treatment of various cancers.

• • Thermal Stability (DSC, DSF) Analysis of Peptide Drugs

  Peptide drugs are biologically active molecules composed of multiple amino acids linked by peptide bonds. Most of them are derived from endogenous or natural peptides, so they have little or no side effects on the human body. In comparison to protein drugs, peptide drugs have advantages such as good stability, high purity, low production cost, and low immunogenicity.

• • Peptide Coverage Analysis of Recombinant Protein Drugs

  Recombinant protein drugs refer to protein products derived from animals and plants through biotechnological research and development, which have certain biological activity and can prevent and diagnose diseases in humans, animals, and plants. Compared to small molecule drugs, recombinant protein drugs have advantages such as high activity, high specificity, and low toxicity, which have attracted extensive attention from researchers.

• • Protein Cross-Linking Guide: From Sample Prep to Interaction Analysis

  Protein cross-linking experiment is a commonly used technique to study the interaction between proteins.