Resources
Proteomics Databases
Metabolomics Databases
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• LC-MS/MS Liquid Chromatography Mass Spectrometry
LC-MS/MS Liquid Chromatography Mass Spectrometry is a technology that integrates Liquid Chromatography (LC) with Tandem Mass Spectrometry (MS/MS). Liquid Chromatography is responsible for separating the components of a mixture, while Mass Spectrometry detects and identifies the molecular weight and structural information of these components. By combining these two techniques, LC-MS/MS achieves high sensitivity and specificity in complex analyses. This method has found widespread applications in variou......
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MS protein sequencing is a technology used for the identification and quantitative analysis of proteins. It determines the amino acid sequence of proteins by analyzing their mass spectrometry data, which provides insights into their structure and function. In disease research, this technology enables the identification of disease-relevant protein biomarkers and aids in uncovering the molecular mechanisms underlying various diseases. These capabilities hold promise for advancing early diagnosis and per......
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• MALDI-TOF MS Bacterial Identification
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Bacterial Identification (MALDI-TOF MS Bacterial Identification) is an advanced technology for microbial identification, offering rapid and accurate bacterial species identification. MALDI-TOF MS Bacterial Identification efficiently classifies and identifies bacteria by analyzing their protein spectra. Compared to traditional biochemical methods, MALDI-TOF MS significantly reduces time and resource consumption, typically prov......
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• Mass Spectrometry in Protein Sequencing
Mass spectrometry in protein sequencing is a technique used to analyze the amino acid sequence of proteins. This method offers detailed structural information about proteins by measuring the mass-to-charge ratio (m/z) of their fragments. With the advancement of genomics, scientists have realized that relying solely on genomic sequences is insufficient to fully understand the complex functions of organisms. Proteins, being the direct executors of cellular functions, are better understood through mass s......
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• Label-Free Quantification Proteomics
Label-free quantification proteomics is an advanced technique used to study protein expression levels and dynamic changes within biological systems. Unlike traditional labeling methods, this technique does not rely on isotopic or chemical labels, but instead directly measures the relative abundance of proteins in a sample using mass spectrometry. This method offers significant advantages for the relative quantification of proteins in complex biological samples, especially in situations involving limit......
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Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis (MALDI-TOF MS Analysis) is a powerful technique used to analyze macromolecules such as proteins, peptides, and polysaccharides. In proteomics, MALDI-TOF MS Analysis is employed to identify and characterize proteins in complex biological samples. By analyzing peptide mass fingerprints, the sequence and structure of proteins can be precisely determined, providing valuable information for disease research and drug devel......
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• Protein Quality Evaluation Methods
Protein quality evaluation methods are essential tools for assessing the nutritional value, bioavailability, and functional properties of proteins. These methods provide critical information for determining how well proteins meet dietary requirements and their potential for use in various food, pharmaceutical, and clinical applications. Current common protein quality evaluation methods include the Chemical Score, Amino Acid Score (AAS), Protein Digestibility-Corrected Amino Acid Score (PDCAAS), Protei......
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Impurity profiling involves identifying, quantifying, and managing non-target components present in samples across disciplines like chemistry, pharmaceuticals, and biotechnology. These impurities may include by-products, degradation products, residual solvents, or other foreign substances from synthesis processes. The objective of impurity profiling is to ensure the sample's purity and stability, thus enhancing the research outcomes' accuracy and reliability. This technique finds applications from bas......
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• High-Performance Liquid Chromatography-Tandem Mass Spectrometry
High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) is a sophisticated analytical technique that integrates high-performance liquid chromatography (HPLC) with mass spectrometry (MS) to separate, identify, and quantify target molecules in complex samples. HPLC is utilized for the efficient separation of diverse components within a sample, while MS provides highly sensitive and selective detection through ionization and analysis of the mass-to-charge ratio (m/z) of molecules. Ta......
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Native mass spectrometry is a critical analytical technique for investigating the complex structures and interactions of proteins. Unlike traditional denaturing mass spectrometry, this approach avoids using harsh conditions, such as strong acids, strong bases, or elevated temperatures, which can disrupt the tertiary structure of proteins. This allows researchers to examine proteins and their complexes in a state close to their natural physiological conditions. Native mass spectrometry is widely applie......
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