Resources

Proteomics Databases



Metabolomics Databases

• • Protein Sequence Analysis Overview

  Protein sequence analysis involves determining the amino acid sequence and chemical modifications of proteins using advanced methodologies. Proteins are essential macromolecules that adopt complex three-dimensional structures derived from their linear amino acid sequences, enabling them to perform diverse cellular functions. The primary objective of protein sequence analysis is to elucidate the primary structure of proteins, providing critical insights into their function, structure, and interactions.......

• • Protein Sequencing by Mass Spectrometry

  Protein sequencing by mass spectrometry is a powerful method for determining the amino acid sequence of proteins. Proteins play central roles in biological processes, with their structure and function directly influencing an organism's growth, development, and metabolism. Understanding the amino acid sequence of proteins provides crucial insights into their functional mechanisms. Traditional methods such as Edman degradation, while accurate, have been largely supplanted by mass spectrometry-based tech......

• • Protein Characterization Techniques

  Protein characterization techniques comprise a diverse array of methodologies that facilitate in-depth understanding of protein structure, function, and interactions. Key techniques include mass spectrometry, nuclear magnetic resonance (NMR) spectroscopy, X-ray crystallography, circular dichroism (CD), and cryo-electron microscopy (Cryo-EM). Mass spectrometry is indispensable in the identification and quantification of proteins, particularly within proteomics, enabling the rapid and precise analysis o......

• • Protein Purification Size Exclusion Chromatography

  Protein purification size exclusion chromatography (SEC), also known as gel filtration chromatography, is a technique widely used to separate proteins and other biological macromolecules based on their size and shape. In proteomics, precise separation and purification of target proteins are essential for elucidating their structure and function. SEC relies on a porous medium packed within a chromatography column, allowing molecules to traverse different pathways based on their size. Smaller molecules ......

• • Peptide Content Determination

  Peptide content determination refers to the precise measurement of peptide levels in a sample using scientific methods. This process allows researchers to accurately assess the concentration and distribution of peptides in a sample, providing vital data for product quality control, clinical treatment planning, and new drug development. As fragments of proteins, peptides possess unique biological activities and are commonly used in early disease diagnosis, therapeutic drug development, and functional i......

• • Protein Characterization Methods

  Protein characterization methods encompass a range of techniques, including mass spectrometry, electrophoresis, X-ray crystallography, and nuclear magnetic resonance (NMR) spectroscopy. These methods are essential for understanding protein structure and function in various biological contexts. Mass spectrometry enables precise determination of protein molecular weight and structural features, and is widely utilized in proteomics research. Electrophoresis, on the other hand, is a common method for prot......

• • Protein Purity Analysis

  Protein purity analysis refers to the process of determining and evaluating the purity of protein samples using various techniques. In the fields of biochemistry and molecular biology, the purity of proteins directly impacts the accuracy and reproducibility of experimental results. Thus, protein purity analysis is vital for any research or application involving proteins. The main purpose of protein purity analysis is to ensure the effectiveness of downstream experiments and to guarantee the safety and......

• • Peptide Content Analysis

  Peptide Content Analysis refers to the measurement of peptide compounds in samples, used for quantitatively analyzing the concentration and composition of peptides in the sample. Peptides are molecules formed by the linkage of amino acids through peptide bonds and are involved in regulating various physiological processes such as hormone secretion, immune responses, and neurotransmission. The biological activity of peptides and their potential in drug development have made peptide content analysis an ......

• • Protein Content Analysis Method

  Protein content analysis methods are crucial for diverse fields, including food science, biotechnology, and pharmacology. These methods, including Kjeldahl nitrogen determination, colorimetric assays, UV absorption, HPLC, and mass spectrometry, each have distinct strengths and applications tailored to specific research needs. For instance, the Kjeldahl method remains a gold standard in food industries for measuring total protein content by analyzing nitrogen levels in complex samples. Meanwhile, col......

• • Protein Content Determination

  Protein content determination refers to the precise measurement of protein concentration and content in a sample. Numerous methods are available for protein content determination, each with distinct advantages and limitations. The most commonly used methods include colorimetric assays, fluorescence techniques, mass-based methods (such as mass spectrometry), and immunological assays (e.g., ELISA). Accurate protein content determination plays a crucial role in evaluating the nutritional value of f......