Resources

Proteomics Databases



Metabolomics Databases

• • Advantages and Disadvantages of 2D Blue Native/SDS-PAGE Protein

  2D Blue Native/SDS-PAGE (2D BN/SDS-PAGE) is a widely used technique for studying protein complexes. This method combines the strengths of Blue Native Polyacrylamide Gel Electrophoresis (Blue Native PAGE) and SDS-PAGE, providing detailed information on the assembly status and components of protein complexes. The following section discusses the advantages and disadvantages of this technique in detail.

• • Principle of 2D Blue Native/SDS-PAGE Protein Complex Analysis

  In biochemical and molecular biological research, analyzing protein complexes is crucial for understanding many physiological and pathological processes in organisms. 2D Blue Native/SDS-PAGE (Two-Dimensional Blue Native/SDS-Polyacrylamide Gel Electrophoresis) is a powerful technique for separating and analyzing protein complexes. This article will detail the principles of 2D Blue Native/SDS-PAGE complex analysis.

• • Mechanism of 2D Gel Electrophoresis Image Analysis

  Two-dimensional electrophoresis (2-DE) is a crucial technique in proteomics research used for separating and analyzing complex protein mixtures. It combines isoelectric focusing (IEF) and SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) to separate protein samples, followed by image analysis for quantitative and qualitative assessment of the separation results. This article details the mechanism of two-dimensional electrophoresis image analysis.

• • Workflow of 2D Gel Electrophoresis Image Analysis

  Two-dimensional gel electrophoresis (2-DE) is a high-resolution protein separation technique widely used in proteomics research. It combines isoelectric focusing (IEF) and SDS-PAGE electrophoresis, allowing the separation of complex protein mixtures in two-dimensional space. The analysis of 2-DE images is a crucial part of this technique, enabling quantitative and qualitative understanding of protein expression in samples. The following is the workflow of 2-DE image analysis.

• • Application of 2D Gel Electrophoresis Image Analysis

  Two-Dimensional Electrophoresis (2-DE) is a high-resolution technique for protein separation, facilitating the efficient analysis of complex protein mixtures. Since its invention, 2-DE has found extensive applications in biological research, medical diagnostics, and biotechnology. This article discusses the applications of two-dimensional electrophoresis image analysis, emphasizing its significance in proteomics, disease research, and drug development.

• • Advantages and Disadvantages of 2D Gel Electrophoresis Image

  Two-dimensional electrophoresis (2D electrophoresis) is a widely used technique in proteomics research. By combining isoelectric focusing (IEF) and SDS-polyacrylamide gel electrophoresis (SDS-PAGE), it separates protein samples in two dimensions. This article discusses the advantages and disadvantages of 2D electrophoresis image analysis in detail.

• • Principle of Two-Dimensional Electrophoresis Image Analysis

  Two-dimensional electrophoresis (2-DE) is a fundamental technique in proteomics research, widely utilized for protein separation and identification. This technique hinges on the distinct migration rates of proteins under varying electrophoretic conditions, involving two sequential electrophoretic separations. Two-dimensional electrophoresis image analysis is pivotal, allowing for the quantitative and qualitative assessment of protein mixtures.

• • Mechanism of Protein Immunoblotting and Electrotransfer

  Protein immunoblotting (Western blotting) is a widely used experimental technique in molecular biology, cell biology, and biochemistry research. This technique combines electrophoresis, membrane transfer, and immunodetection methods to detect the presence and quantify specific proteins. This article provides a detailed explanation of the working principle of protein immunoblotting, with a particular focus on the mechanism of electrotransfer.

• • Application of Protein Immunoblotting and Electrotransfer

  Protein immunoblotting (Western blotting) and electrotransfer are indispensable techniques in biological research. They are extensively used for protein identification, expression level analysis, and interaction studies. Due to their precision and sensitivity, these techniques are invaluable across various research fields, including medicine, biochemistry, molecular biology, and drug development. This article explores the applications of protein immunoblotting and electrotransfer.

• • Workflow of Protein Immunoblotting and Electrotransfer

  Protein immunoblotting, commonly known as Western blotting, is a widely utilized experimental technique designed to detect and analyze the presence, expression levels, and molecular weight of specific proteins. The methodology encompasses six primary steps: protein extraction, protein electrophoresis, protein transfer, blocking, antibody incubation, and signal detection. This article provides an in-depth overview of each step within this workflow.