Resources

Proteomics Databases



Metabolomics Databases

• • Principle of Protein Analysis

  Protein analysis is a fundamental aspect of biochemical and biopharmaceutical research, providing insights into the structure, function, and interactions of proteins. Proteins are crucial macromolecules involved in virtually all cellular processes, and their analysis can reveal information essential for understanding biological mechanisms, disease progression, and therapeutic development.

• • Mechanism of 2D Blue Native/SDS-PAGE Protein Complex Analysis

  2D Blue Native/SDS-PAGE complex analysis is a sophisticated technique employed to elucidate the structure and function of protein complexes. This method synergizes Blue Native PAGE with Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE), facilitating the separation and analysis of protein complexes from complex biological samples through a sequential two-step process. This article explicates the fundamental principles and mechanisms underlying 2D Blue Native/SDS-PAGE.

• • Application of 2D Blue Native/SDS-PAGE Protein Complex Analysis

  Protein complexes play crucial roles in physiological and pathological processes within cells. Understanding their composition and structure is essential for investigating biological processes and developing therapies. Two-Dimensional Blue Native/SDS-PAGE (2D BN/SDS-PAGE) is a powerful technique for analyzing protein complexes.

• • Workflow of 2D Blue Native/SDS-PAGE Protein Complex Analysis

  2D Blue Native/SDS-PAGE complex analysis is a robust technique for separating and analyzing protein complexes. It combines Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) with subsequent SDS-PAGE, providing detailed information about the native state and components of protein complexes in the same gel. This method is invaluable for elucidating protein interactions, complex structures, and functions.

• • N-Glycan Profiling and Its Analysis Workflow

  N-glycan analysis is a method used to analyze glycan structures, especially when studying glycosylation on proteins or other biomolecules. Glycosylation (or glycation) is a common mode of biomolecular modification in cells, playing a key role in protein stability, activity, cell signaling, cell-cell interaction, and more.   N-glycan refers to the glycan chain attached to the asparagine nitrogen, which is a type of N-linked glycoprotein.

• • Ubiquitination Identification Process of Tau Protein

  Tau protein is a microtubule-associated protein, mainly present in nerve cells, and participates in the stabilization of microtubules. In certain neurodegenerative diseases, such as Alzheimer's disease (AD), Tau protein may undergo abnormal phosphorylation and aggregation, forming so-called Tau tangles or neurofibrillary tangles.   Ubiquitination is a kind of post-translational modification of proteins, involving the linkage of one or more ubiquitin molecules to the target protein.

• • DIA and SWATH

  SWATH (sequential window acquisition of full theoretical mass spectra) and DIA (data independent acquisition) are two mass spectrometry techniques mainly used in proteomics research. They are both data-independent acquisition strategies, which are different from traditional data-dependent acquisition (DDA, Data-Dependent Acquisition) methods.   SWATH (Sequential Window Acquisition of All Theoretical Mass Spectra)

• • Peptide Spectrum Analysis

  Peptide spectrum analysis is a key concept in mass spectrometry, mainly used for the identification of proteins and their degradation products - peptides. In mass spectrometry analysis, protein samples are first hydrolyzed into smaller peptides by enzymes (such as trypsin), and then these peptides are analyzed by mass spectrometry.

• • Co-Immunoprecipitation Detection of Protein Ubiquitination

  Co-immunoprecipitation (Co-IP) is an experimental technique used to detect the interaction between a protein and other proteins. When it comes to detecting protein ubiquitination, Co-IP can be used to determine whether the target protein is ubiquitinated, and its interaction with ubiquitin protein.

• • What Is the Concept of Proteomics?

  Proteomics is a rapidly advancing field within molecular biology that focuses on the large-scale study of proteins, particularly their structures and functions. The term "proteome" refers to the entire set of proteins produced or modified by an organism or system. Proteomics encompasses various techniques to analyze the proteome, aiming to understand protein functions, interactions, and their role in biological processes.