Resources
Proteomics Databases
Metabolomics Databases
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• Mass Spectrometry Molecular Weight
Mass spectrometry molecular weight determination is a technique used to determine the molecular weight of a molecule or ion by measuring its mass. This method is widely applied in fields such as biological sciences, chemistry, and materials science, where it aids researchers in determining the molecular weight of biomolecules and inferring their molecular structures. The technique relies on the use of a mass spectrometer, a highly sensitive and complex instrument capable of quickly and accurately iden......
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• Intact Mass Analysis of Antibodies
Intact mass analysis of antibodies is an analytical process used to comprehensively assess the structural integrity and consistency of antibody molecules in terms of their quality and structure. This analysis is essential in the development of antibody therapeutics, production quality control, and functional studies. With the growing use of antibody-based drugs in the treatment of cancer, autoimmune diseases, and infectious diseases, intact mass analysis of antibodies has become a central component of......
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• Analysis of Biomolecules Using Mass Spectrometry
Mass spectrometry is an efficient analytical technique extensively used in biomolecular research. The analysis of biomolecules using mass spectrometry involves measuring the mass-to-charge ratio of ions within a sample, providing researchers with the ability to elucidate complex biomolecular structures, identify compounds, and quantitatively analyze their concentrations. This method enables scientists to gain detailed insights into dynamic changes within biological systems at the molecular level. Ma......
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• Analysis of Protein Complexes Using Mass Spectrometry
Analysis of protein complexes using mass spectrometry is a crucial technique for the precise quantitative and qualitative study of biomolecules. Mass spectrometry provides high sensitivity and accuracy, making it an indispensable tool for exploring protein networks and interactions. By analyzing the mass-to-charge ratios (m/z) of ionized molecules, mass spectrometry allows for both the identification and quantification of proteins. The study of protein complexes is fundamental for understanding cellul......
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• Determining Molecular Weight from SDS PAGE
Determining molecular weight from SDS PAGE is one of the most commonly used analytical techniques in protein research. This method leverages the electrophoretic properties of proteins, separating them in polyacrylamide gel to estimate the relative molecular weight of the target protein. The process relies on the fact that SDS treatment causes proteins to lose their native conformation, existing instead as linear molecules, making molecular weight the primary determinant of migration rate. The princi......
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Molecular mass spectrometry is a widely used technique for analyzing molecular mass and structure, with applications across various fields, including chemistry, biology, and medicine. By measuring the mass of ionized samples, it provides detailed information on molecular mass, chemical composition, and structure. Due to its high sensitivity and resolution, molecular mass spectrometry has become one of the most critical tools in modern analytical chemistry. The principle of molecular mass spectrometr......
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• Determination of Molecular Weight by SDS PAGE
The determination of molecular weight by SDS PAGE is a widely used technique in protein research, particularly for assessing the molecular weight of proteins and peptides. This method utilizes electrophoretic separation, where proteins migrate in an electric field based on their molecular size, allowing the inference of their molecular mass. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a powerful method for separating protein samples based on their molecular size. The tech......
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• Determination of Molecular Weight of Protein by SDS PAGE
The determination of molecular weight of protein by SDS PAGE is a widely used technique in the fields of biochemistry and molecular biology. SDS-PAGE, or sodium dodecyl sulfate-polyacrylamide gel electrophoresis, is primarily used for protein separation. By comparing the migration distances of proteins in a sample with those of known molecular weight markers, this method enables the estimation of the molecular weight of proteins. SDS-PAGE is known for its simplicity, high resolution, and essential rol......
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The peptide mapping procedure is a critical process in both proteomics and biopharmaceutical research. By enzymatically digesting, separating, and analyzing protein or antibody molecules using mass spectrometry, this procedure generates precise peptide maps. These maps reveal structural features, post-translational modifications, and potential impurities or variants, providing accurate data for drug development, quality control, and basic research. The scientific rigor and accuracy of this method are ......
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Protein stability assays are critical techniques used to assess a protein's ability to maintain its native structure and function under specific conditions. The stability of a protein is a key determinant of its biological activity, storage conditions, and functional lifespan. These assays are widely applied in fields such as biopharmaceuticals, structural biology, and food science. By conducting precise protein stability assays, researchers can optimize protein formulations, ensure the stability of b......
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