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Proteomics Databases



Metabolomics Databases

• • Gel Permeation Chromatography for Molecular Weight Determination

  Gel permeation chromatography (GPC) is a widely used separation technique for determining the molecular weight and distribution of macromolecules. This method relies on the permeation and exclusion effects of molecules within a porous stationary phase, where separation is achieved based on differences in molecular volume. Due to its efficiency, rapidity, and stability, gel permeation chromatography for molecular weight determination has found extensive applications in polymer science, biochemistry, an......

• • MALDI-TOF Peptide Mass Fingerprinting

  MALDI-TOF Peptide Mass Fingerprinting (MALDI-TOF PMF) offers a rapid and highly accurate method for identifying and quantifying protein compositions in complex samples by detecting peptide masses and their relative abundances.   MALDI-TOF peptide mass fingerprinting combines matrix-assisted laser desorption/ionization (MALDI) with time-of-flight mass spectrometry (TOF-MS). Laser-induced desorption and ionization of peptides generate charged peptide ions from the sample. These ions are then analyz......

• • Molecular Weight Determination by Gel Filtration Chromatography

  The principle of molecular weight determination by gel filtration chromatography relies on the differential diffusion rates of molecules within gel-filled matrices. Larger molecules, unable to penetrate the pores of gel particles, elute earlier from the chromatography column. Conversely, smaller molecules diffuse into the gel pores, resulting in longer retention times and delayed elution. By accurately measuring the sample's elution time (or volume), the molecular weight can be determined. This proces......

• • Determination of Molecular Weight by Mass Spectrometry

  Determination of molecular weight by mass spectrometry is a highly precise analytical technique used to measure the molecular weight of substances such as proteins, antibodies, and other biopharmaceuticals. The method relies on ionizing the sample and separating the resulting ions according to their mass-to-charge ratios (m/z) to accurately determine their mass. This technique has wide-ranging applications in fields such as chemistry, biology, and medicine.   The process of determination of molecular ......

• • DLS Molecular Weight Determination

  DLS molecular weight determination is an analytical technique used to measure the size and distribution of particles or molecules in solution. This method is based on the fluctuations in light scattering caused by the Brownian motion of particles in the solution. By analyzing these fluctuations, specifically the changes in the autocorrelation function, the size and molecular weight of the particles can be inferred. DLS molecular weight determination is widely employed in various fields, including chem......

• • Molecular Weight Determination by Light Scattering Method

  Molecular weight determination by light scattering method is a technique used to determine the molecular weight of macromolecules or particles by analyzing the changes in scattered light intensity. The method is based on the interaction between light and matter: when light passes through a solution, the molecules scatter the light, with the intensity of scattering being closely related to both the molecular weight and concentration. By analyzing the angular distribution and intensity of scattered ligh......

• • Determination of Molecular Weight by Light Scattering

  Determination of molecular weight by light scattering is an essential technique for accurately determining the molecular weight of macromolecules such as proteins, polymers, and other biopharmaceuticals. This method is non-destructive, highly precise, and rapid, making it a valuable tool for investigating the physicochemical properties of large molecules.   In this process, determination of molecular weight by light scattering involves irradiating the sample with a laser and detecting the intensity fl......

• • Analytical Ultracentrifugation Molecular Weight Determination

  Analytical ultracentrifugation molecular weight determination is an advanced technique used to measure the molecular weight of macromolecules and their complexes. This method is highly accurate and reliable for analyzing proteins, nucleic acids, and other biological macromolecules. By generating centrifugal forces through ultra-high-speed rotation, it enables the separation and analysis of particles based on their sedimentation velocity, which is directly related to their molecular weight and shape. A......

• • Ultracentrifugation Molecular Weight Determination

  Ultracentrifugation molecular weight determination is a powerful analytical method used to determine the molecular weight of macromolecules such as proteins, nucleic acids, polymers, and other biological macromolecules. By applying high centrifugal forces, this technique separates molecules based on their sedimentation coefficients and molecular weights, enabling quantitative analysis of the sample.   The principle of ultracentrifugation molecular weight determination is based on the sedimentation beh......

• • Intact Mass Analysis Proteins

  Intact mass analysis proteins is a widely utilized technique for determining the molecular mass of proteins in their native state, without requiring prior degradation or fragmentation. This approach is essential in biopharmaceuticals and life sciences, as it provides precise information on protein integrity, post-translational modifications (PTMs), and isomer distributions. Its ability to analyze proteins in their intact form makes it a cornerstone of advanced protein characterization.   Principles of......