Resources

Proteomics Databases



Metabolomics Databases

• • Birdshot Proteomics Sequencing

  Protein sequencing is the determination of the primary structure of proteins, which is the sequence of amino acids that make up the protein. The primary structure of a protein largely determines its spatial structure and biological functions. Currently, commonly used protein sequence analysis methods mainly include terminal sequencing methods (such as Edman degradation method and carboxypeptidase method) and mass spectrometry-based methods. Shotgun is a mass spectrometry-based protein sequencing method.

• • Proteomics Technology 4D

  In 2018, 4D proteomics officially entered the public view. Compared to traditional 3D proteomics, 4D proteomics adds the concept of ion mobility, in addition to retaining retention time, m/z, and intensity, thereby significantly improving scanning speed and detection sensitivity. Ion mobility, also known as ion drift rate, refers to the speed at which an ion passes through a certain gaseous area under unit electric field strength, which is often related to the shape, size, and charge state of the ion.

• • Detection of Histone Lactylation Modification

  Histone acetylation is a widespread post-transcriptional modification in eukaryotes that plays a crucial role in various biological processes, including regulation of gene expression, cell cycle control, and environmental stimulus response. Therefore, research on histone acetylation modifications has significant implications for a deeper understanding of physiological and pathological processes in organisms.

• • Does DSF Detection Require Protein Purification?

  Differential Scanning Fluorimetry (DSF), also known as melt curve analysis or thermal stability assay, is a commonly used technique for assessing protein stability and screening drug targets. DSF determines the stability of a protein by measuring its melting temperature (Tm) as the temperature rises. Melting occurs when the protein transitions from a structurally stable state to a disordered state. The Tm value of a protein can serve as an important indicator of its stability.

• • Plant Stem Vein 2D-DIGE Quantitative Proteomics

  The vascular bundles of plants are their lifelines, responsible for transporting water and nutrients to the entire plant body. In this complex transportation system, proteins play a central role, supporting various biological functions of stem veins. To deeply understand how proteins in the stem veins interact with plant growth, development, and adaptability, the 2D-DIGE quantitative proteomics technique was born.

• • Protein Sulfation Mass Spectrometry Identification

  Sulfation is a potent post-translational modification (PTM) that plays a crucial role in cellular biology. However, the identification and quantification of sulfated proteins have always been a challenge due to their low abundance and instability. The development of mass spectrometry technology in recent years has provided us with powerful tools to address this issue. In this article, we will explore the mass spectrometric identification of sulfated proteins.

• • Mass Spectrometry Ubiquitination Molecular Weight

  Ubiquitination modification is one of the most common forms of post-translational modification in biological molecules, playing a key role in regulating protein homeostasis, cell cycle and immune response, among other biological processes. Therefore, accurately quantifying and identifying ubiquitination modification sites is crucial for understanding the molecular mechanisms of these biological processes.

• • Mass Spectrometry Method for the Detection of HCP Residues

  Mass spectrometry is a highly sensitive qualitative and quantitative analysis method that has developed rapidly in recent years. Its application fields include biopharmaceuticals, environmental monitoring, food safety testing, and many other fields. In the field of biopharmaceuticals, it has a unique advantage in the analysis of proteins and peptide drugs. This article mainly discusses the application of mass spectrometry in the detection of host cell protein (HCP) residues.

• • Qualitative Lactic Acid Modified Protein Composition

  Lactylation is an important protein post-translational modification that regulates the structure and function of numerous proteins within the cell.

• • Protein Mass Spectrometry Sequencing Result Filtering

  Protein mass spectrometry is a vital tool for analyzing protein expression, modification, and interaction. However, protein mass spectrometry data typically contain a large amount of noise, challenging the protein identification accuracy. Hence, filtering mass spectrometry sequencing results is of great significance.