Resources

Proteomics Databases



Metabolomics Databases

• • Gorilla Tears O-Glycosylation Site Analysis

  Gorillas, as close relatives of humans, their physiological, genetic, and behavioral characteristics have always been hot topics in the field of research. Especially the tears of gorillas, have shown in recent years in several studies that they have special biochemical and bioactive properties.

• • Serum Ionomic Analysis

  Understanding and analyzing the composition of human biofluids has become crucial for revealing disease mechanisms, monitoring health status, and discovering new biomarkers.

• • Metallic Elements in Cardiac Tissue

  The metal elements in cardiac tissue, such as calcium, magnesium, iron, and zinc, are essential elements for the formation of cardiac cells and tissues, and are also necessary components for the maintenance of normal cardiac functions. Their quantity and distribution have a critical impact on the normal functions of the heart. Any excess or deficiency of metal elements can lead to the occurrence of heart diseases.

• • Peptide Drug Impurity Detection

  Peptide-based drugs are a special type of drugs that fall between traditional chemical drugs and large molecule drugs. In recent years, due to their features such as low toxic side effects, strong biological activity, no metabolic heterogeneity, and low dosage, they are receiving increasing attention in the medical field. The production of peptide drugs primarily adopts three methods: chemical synthesis, animal tissue extraction, and gene recombination expression.

• • Ways to Detect HCP Coverage Rate

  HCP (Host Cell Proteins) coverage analysis is an important analytical method in the field of biopharmaceuticals, used to evaluate the detection coverage range and clearance efficiency of host cell proteins in biologic drugs. This analysis is crucial for ensuring the safety and purity of biopharmaceutical products. The following are several commonly used methods for HCP coverage analysis:

• • Where Can I Download Data on Proteomics?

  1. PRIDE Database PRIDE (The PRoteomics IDEntifications database) is a bioinformatics database maintained by the European Bioinformatics Institute (EBI) that provides a wealth of proteomics data. This data can be freely downloaded from the official PRIDE website. To download data from PRIDE, first visit their official website. Then, enter the keywords of the protein or experiment you are interested in the search bar.

• • Ubiquitin Detection by Immunoprecipitation Band

  Ubiquitination is a post-translational modification process that involves various biological processes, including signal transduction, cell cycle regulation, cell stress responses, and cell apoptosis. It plays a crucial role in maintaining protein homeostasis, regulating various biological reactions in cells, and ensuring the normal physiological functions of organisms. Immunoprecipitation is a commonly used method for detecting protein interactions.

• • Process of IP Mass Spectrometry

  The process of IP-MS (Immunoprecipitation Tandem Mass Spectrometry Analysis) mainly includes the following steps.

• • Perform Protein Mass Spectrometry After IP

  Immunoprecipitation (IP) is a method of purifying proteins. The principle is to incubate the antibody of the target protein with the sample extract, allowing the antibody and protein to bind in the solution. Then the antibody/antigen complex is extracted from the sample with agarose gel coupled with bacterial protein A/G. IP mainly captures target proteins and their interacting proteins selectively. If you want to quantify these proteins, you need to cooperate with other technologies.

• • The Application of Shotgun in Proteomics

  In proteomics, the Shotgun mass spectrometry method is a commonly used technique for identifying and quantifying proteins in complex samples. This method digests protein samples into small fragments (such as peptides) and then analyzes them using a mass spectrometer to obtain information on the proteome.