Resources

Proteomics Databases



Metabolomics Databases

• • What Is Peptide Mass Spectrometry Identification

  Peptides are a class of compounds whose molecular structure lies between amino acids and proteins, composed of 20 natural amino acids in different compositions and arrangements, from dipeptides to complex linear or cyclic structures of polypeptides. We refer to peptides composed of 2-10 amino acids as oligopeptides, those composed of 11-50 amino acids as polypeptides, and those composed of more than 50 amino acids as proteins.

• • How to Determine the Extinction Coefficient

  The extinction coefficient, also known as the molar absorptivity or absorption coefficient, refers to the absorbance value of the test solution to light. As an important parameter of optical properties, its measurement is crucial for understanding the structure, properties, and functions of materials.

• • Protein Thermal Stability Analysis

  Protein thermal stability refers to the ability of a protein polypeptide chain to change shape under the influence of temperature, mainly reflected in the unique chemical properties and spatial conformation changes of the polypeptide chain when the temperature changes. The smaller the deformation, the higher the thermal stability. In the fields of biotechnology, drug research and development, and the food industry, protein thermal stability analysis is of great significance.

• • Spatial Proteomics

  Spatial proteomics is a discipline that studies the three-dimensional spatial distribution of proteins within cells and their interactions with other molecules. Different from traditional proteomics which mainly focuses on protein sequences, expression levels, and interactions, spatial proteomics pays more attention to the spatial location and dynamic changes of proteins within cells.

• • Mass Spectrometry Flow Cytometry Technology

  Flow Cytometry is the most classic single-cell analysis technique, which can detect multiple parameters in single cells, thus conducting subgroup and functional analysis of samples. Traditional flow cytometry is generally based on the detection of fluorescence emission spectra. Since the emission spectra of fluorescent groups are relatively short, overlap often occurs between the emissions of different fluorophores during multi-parameter detection.

• • Detecting Glycosylation

  Glycosylation of protein is a widespread, structurally complex and varied post-translational modification of proteins, playing important roles in cells and organisms, such as participating in the regulation of cell recognition, adhesion, signal transduction, etc.

• • Extracellular Vesicle Proteomics

  Exosomes are small vesicles with a lipid bilayer membrane, with diameters ranging from 30 to 150 nm. They are secreted by living cells and are widely present in various body fluids, including blood, saliva, urine, synovial fluid, milk, cerebrospinal fluid, etc. Exosomes are rich in a large number of bioactive molecules, such as nucleic acids, proteins, lipids, and sugars.

• • Acetylation Detection Methods

  Protein acetylation is a critical protein post-translational modification, which involves the covalent binding of an acetyl group to a lysine residue by an acetyl donor (such as acetyl coenzyme A) through enzymatic or non-enzymatic means. Currently, two forms of acetylation are known - Nα acetylation and Nε acetylation.

• • Protein Modification Prediction Software

  Protein modification is a crucial biochemical process in living organisms. To boost the efficiency of protein modification research, scientists have developed various protein modification prediction software. These tools are primarily used to predict potential modification sites on proteins and possible functional changes post-modification.

• • Peptide Identification Process

  Peptide identification is a core process in proteomics, involving the analysis of proteins in biological samples to identify specific peptide segments. This process is not only crucial for basic biological research, but it also plays a key role in disease diagnosis, drug discovery, and biomarker identification. A complete peptide identification process can be approximately divided into the following stages.