Resources

Proteomics Databases



Metabolomics Databases

• • Is Prokaryotic Expression Used Before MS Protein Modification?

  In biological research, mass spectrometry analysis is a widely used technique for the quantitative and qualitative analysis of proteins and their modifications. Understanding protein modifications is crucial to reveal their functions. However, the study of protein modifications often faces challenges in preparing suitable protein samples.

• • Proteomics and 2-Hydroxyisobutyrylation MS Analysis

  Proteomics is a discipline that studies all protein expression, structure, and function. It involves describing protein sequences, protein structures, protein interaction networks, and the biological functions and functional dynamics of proteins. Research in proteomics helps to understand the occurrence and development of diseases, reveal the molecular mechanisms of diseases, and provide new drug targets and biomarkers.

• • Circular Dichroism for Determining Absolute Configuration

  The absolute configuration is a way of describing stereochemical molecules, defined according to the spatial configuration of stereoisomers. This is a method of categorizing based on the actual three-dimensional distribution of the structure, unrelated to optical activity or flocculation activity. The most commonly used description method is the Cahn-Ingold-Prelog (CIP) rules.

• • Protein Quantification via Coomassie Brilliant Blue Assay

  The Coomassie Brilliant Blue method for determining protein content is a commonly used method in biology and chemistry experiments. The basic principle is to use Coomassie Brilliant Blue under acidic conditions to stain the structure of proteins, and then determine the protein content in the sample through the ultraviolet spectrophotometric method.

• • N-Glycan Structure Analysis

  Glycobiology is an important part of life science, studying the structure and function of sugar molecules (also known as polysaccharides or glycosides) in biology. Among them, N-glycans are a very important type of sugar molecule, widely present in organisms, and involved in various biological processes, such as intercellular communication, pathogen infection, and immune response.

• • How to Interpret Peptide Graphics?

  Peptide mapping is a representation of protein sequences and is widely used for qualitative and quantitative analysis of peptides and proteins. Through peptide mapping, we can clearly see the amino acid composition in the protein sequence and the relative positions of each amino acid. However, interpreting peptide maps may be challenging for non-specialists.

• • Mass Spectrometry Cannot Identify the Target Protein

  In biological research, mass spectrometry is an important way to identify and analyze proteins. However, researchers may encounter situations where the target protein cannot be identified by mass spectrometry. This article will discuss the possible reasons and targeted solutions.  

• • Mass Spectrometry-Based Glycan Profiling Analysis

  Glycotype is a type of biological marker that plays a vital role in many biological processes, such as cell adhesion, immune recognition, and virus invasion. However, the structure of glycotype is extremely complex, so its accurate structural analysis and identification are of great significance for biomedical research. This article mainly explores the theory, methods, and applications of mass spectrometry in glycotype analysis.

• • What Mass Spectrometry Is Used in Proteomics?

  Proteomics is a systematic science used to study all the expression, structure, and function of proteins. As an important analytical tool, mass spectrometry plays an indispensable role in proteomics research. In particular, Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS), due to its high sensitivity, high throughput, and high resolution, has become the mainstay of modern proteomics.

• • Quantification of Phosphoproteins in Cells

  Protein phosphorylation within cells is an important biological process that plays a crucial role in many biological events, such as signal transduction, cell growth, differentiation, and apoptosis. Therefore, the quantitative measurement of protein phosphorylation in cells has become an essential research method.