Resources
Proteomics Databases
Metabolomics Databases
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N-terminal Edman sequencing, a method established by Pehr Edman in 1950, remains a cornerstone in protein chemistry for determining the amino acid sequence at the N-terminus of proteins. This method enables precise identification and analysis of the N-terminal amino acids, essential for understanding protein function and structure. The technique hinges on the Edman degradation reaction, wherein the N-terminal amino acid is selectively removed from a polypeptide chain and converted into a detectable de......
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• N-Terminal Edman Degradation
N-terminal Edman degradation is a widely adopted protein sequencing technique used for analyzing the amino acid sequences of proteins. Renowned for its precision and reliability, it serves as an indispensable tool in biochemical and molecular biology research. The methodology involves the sequential identification of amino acids from the N-terminus, facilitating the elucidation of intricate protein structures. Introduced by Pehr Edman in 1950, this method enables the identification of amino acids with......
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• Analytical Techniques for Protein Characterization
Analytical techniques for protein characterization involve the comprehensive measurement, description, and interpretation of various protein properties to gain a deep understanding of their structure, function, and related characteristics. This analysis is vital for biochemistry, drug development, and clinical diagnosis. Through protein characterization, researchers can explore the three-dimensional structures of proteins, identify functional domains, and elucidate their mechanisms of action both intr......
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SEC protein analysis is a widely utilized separation technique in proteomics research. The fundamental principle of SEC involves the separation of molecules based on their size as they pass through a gel filtration medium. SEC is effective in the separation and purification of biomacromolecules, such as proteins and nucleic acids, and it plays a crucial role in proteomics research. The primary applications of SEC protein analysis include assessing protein purity, determining molecular weight, analyzin......
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SEC analysis of proteins is a critical chromatographic technique that separates components based on molecular size differences. This separation is accomplished using a stationary phase column packed with porous beads, where the pore size determines the migration speed of molecules. Large molecules, unable to penetrate the pores, elute quickly due to shorter residence times in the stationary phase, whereas smaller molecules enter the pores, resulting in delayed elution times. SEC analysis is essential ......
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SDS-PAGE purity analysis, known as polyacrylamide gel electrophoresis, is a widely utilized technique for the separation and assessment of protein purity based on molecular weight. This method allows researchers to effectively determine protein sample purity, molecular weight, and molecular weight distribution, thereby facilitating further protein analysis. SDS-PAGE is extensively applied in biopharmaceuticals, molecular biology research, and clinical diagnostics. In biopharmaceuticals, the technique ......
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• Post-Translational Modification Analysis
Post-translational modification analysis is pivotal in studying the various chemical modifications proteins undergo after their synthesis. These modifications occur through enzymatic processes and include phosphorylation, acetylation, methylation, and ubiquitination, among others, each playing critical roles in modulating protein function, structure, and cellular localization. Post-translational modification analysis extends beyond basic biological research, finding applications in disease diagnosis, ......
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• Redox Proteomics Insights into Disease Mechanisms
Redox proteomics focuses on the study of protein oxidative states to gain insights into their regulatory roles, functions, activity, and participation in various physiological processes. Proteins are integral to nearly all biological processes, and redox reactions represent one type of these biochemical interactions. By investigating the mechanisms and regulation of proteins involved in redox reactions, redox proteomics uncovers how changes in cellular redox status impact growth, development, aging, a......
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Proteomic analysis service provides a comprehensive study of protein composition, structure, and function at a large scale, assisting researchers, medical institutions, and businesses in understanding proteomic data from biological samples. Focused on the holistic analysis of proteins within cells or tissues under specific conditions, proteomics enables insights into protein roles across biological processes, unveiling disease mechanisms, developing therapeutic strategies, and discovering biomarkers. ......
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iTRAQ labeling (Isobaric Tags for Relative and Absolute Quantitation) employs isotopic tags to facilitate the simultaneous quantitation of up to eight proteomic samples. By chemically tagging peptides, this method allows for precise quantification of protein abundance using mass spectrometry. iTRAQ's high quantification efficiency makes it especially valuable in biomedical research, including the investigation of complex biological systems and disease mechanisms. Its applications span the discovery of......
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