Resources

Proteomics Databases



Metabolomics Databases

• • iTRAQ Quantitative Proteomics

  iTRAQ (Isobaric Tags for Relative and Absolute Quantitation) is an isotope tagging technology for relative and absolute quantitation, extensively used in proteomics research. iTRAQ technology marks proteins or peptides to achieve protein quantification analysis, thereby revealing the dynamic changes of proteins in biological processes.   The core of iTRAQ technology is to use isotope labeling reagents to label protein samples. These labeling reagents consist of two parts: the balance region and the re......

• • The Role of Primary Structure in the Immunogenicity of Biopharmaceuticals

  Biological products are drugs or other biological preparations manufactured using biotechnology, widely used in disease treatment and health improvement. The primary structure plays a crucial role in the immunogenicity research of biological products. The primary structure refers to the amino acid sequence of proteins or polypeptides in biological products, which determines the structure and function of the biological products.

• • LC-MS Proteomics Technical Points From Sample Preparation to Data Analysis

  In the field of biological sciences, the study of proteomics has become an important means to explore the complexity of biological systems. Liquid Chromatography-Mass Spectrometry (LC-MS) is one of the core technologies of proteomics.   Sample Preparation Sample preparation is a critically important step in LC-MS proteomics, as it directly relates to the accuracy and reliability of subsequent analyses. High-quality sample preparation requires meticulous design and operation.

• • Optimizing Mass Spectrometry Quantification Techniques for Protein

  The technology of mass spectrometry detection of proteins has penetrated into the fields of biomedical, food detection, pharmaceutical analysis, etc., such as the analysis of protein content in drugs, or the discovery of disease biomarkers. However, the accuracy and efficiency of this method are still limited by many factors, such as sample processing, matrix effects, etc. Next, let's break down each step and delve into how to optimize the quantitative techniques for mass spectrometry detection of pro......

• • Glycosylation Modification Omics Analysis From Qualitative to Quantitative

  Protein is one of the most important functional molecules in cells, and studying protein modification is of great significance for a deeper understanding of its biological function. Among them, glycosylation modification, as a common and important way of protein modification, has attracted much attention from researchers. Glycomics is a systematic method for studying protein glycosylation modification. Through this method, we can fully understand the types, locations, and quantities of glycosylation m......

• • Optimizing Immunoprecipitation Experiments to Improve the Purity and Specificity of Peptide Samples

  Immunoprecipitation is an important experimental technique used in the field of biopharmaceuticals to study protein interactions. In this process, antibodies bind specifically to target proteins, and the complexes are then separated by precipitation techniques for further analysis. However, due to the complexity and specificity of the samples, immunoprecipitation experiments may face challenges such as low purity and non-specificity.

• • The Importance and Application of P-value Adjustment in Differential Analysis of Proteomics

  Proteomics is a scientific field that studies the complete set of proteins, their structures, and functions in organisms. In proteomics research, differential analysis is a critical step that helps us identify the differences in protein expression between different samples. The p-value is a commonly used statistical indicator in differential analysis, which can evaluate whether the observed difference is statistically significant.

• • Optimizing Sample Preparation Steps for Label-Free Semi-Quantitative Chemical Proteomics

  Label-free semi-quantitative proteomics is an important proteomics method that can quantitatively analyze the differential expression of proteins in cells or tissues. In this process, sample preparation is a key factor affecting the accuracy of the results. Optimizing sample preparation steps can maximize the reliability and repeatability of experiments.   Sample Pretreatment In label-free semi-quantitative proteomics, sample pre-treatment is the first step to consider.

• • The Prospects of Label-Free Proteomics in Disease Research

  Proteomics, as an important branch of the field of bioproduct proteomics, is committed to comprehensively analyzing the composition and function of proteins in cells. Label-free proteomics, as a emerging technology, has emerged in the field of disease research in recent years. Its unique advantages make it a powerful tool for revealing the physiological mechanisms of diseases, discovering new therapeutic targets, and achieving personalized medicine.   Label-free proteomics is a proteomics method that ......

• • Methods and Principles of Membrane Protein Glycosylation Analysis

  Glycosylation of membrane proteins is a crucial post-translational modification process that involves the binding of proteins on the cell membrane with sugar molecules. This modification process plays a significant role in cell signaling, cell recognition, cell adhesion, and protein stability.   Types of Membrane Protein Glycosylation Membrane protein glycosylation can be divided into two types: N-glycosylation and O-glycosylation. N-glycosylation occurs on the nitrogen atoms of amino acids in protein......