Resources
Proteomics Databases
Metabolomics Databases
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Histone analysis represents a crucial aspect of proteomics, focusing on the extensive study of histones and their post-translational modifications (PTMs). Histones, as highly conserved basic proteins, reside within the nuclei of eukaryotic cells and, in association with DNA, constitute chromatin's fundamental unit, the nucleosome. By employing histone analysis, researchers gain insights into chromatin's dynamic architecture, gene expression regulation, and cellular state transformations. PTMs of histo......
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• Enzyme Purification and Characterization
Enzyme purification and characterization are crucial processes in biochemical research, involving the separation, extraction, identification, and analysis of enzymes from complex biological systems. Enzyme purification employs various biochemical techniques to eliminate other biological macromolecules and small molecular impurities, resulting in enzymes with specific activities and properties. The characterization of these enzymes entails a detailed assessment of their physical, chemical, and biologic......
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• Conformational Analysis of Proteins
Conformational analysis of proteins focuses on exploring the tertiary structure and dynamic changes in proteins. Protein conformation refers to the three-dimensional spatial arrangement of atoms within a protein molecule. The tertiary structure is defined as the specific spatial form achieved when a polypeptide chain folds further based on its secondary structure. The stability of this structure is predominantly determined by non-covalent interactions such as hydrogen bonds, ionic bonds, hydrophobic i......
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Capillary gel electrophoresis protein analysis (CE protein analysis) involves the separation and analysis of proteins using capillary electrophoresis technology. By filling capillaries with gel media and applying an electric field, proteins are separated based on differences in charge and size. Protein molecules migrate through the capillary at varying speeds influenced by their size, shape, and charge. The gel medium acts as a sieve, allowing smaller protein molecules to pass through more easily, whi......
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• AUC Protein Characterization
The fundamental principle of AUC protein characterization involves systematically analyzing protein properties using ultracentrifugation techniques, with the goal of more precisely elucidating protein structure and function. This method is particularly useful for determining the dissociation constants of protein complexes, assessing molecular weight and its distribution, and evaluating shape factors. AUC protein characterization by examining their sedimentation behavior under a centrifugal force field......
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• Analyzing Amino Acid Sequences
Analyzing amino acid sequences primarily aims to determine the primary structure of proteins, which is essential for understanding their functions, structural stability, evolutionary relationships, and roles in diseases. For instance, analyzing an enzyme's amino acid sequence can reveal the composition of its active site, thereby elucidating its catalytic mechanism. Amino acids, as the fundamental building blocks of proteins, serve as the foundation for this analysis. By studying their sequences, scie......
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Enzyme characterization is a fundamental concept in biochemistry and proteomics. Enzymes, as biological catalysts, are essential for accelerating biochemical reactions in living organisms, participating in processes like metabolism, signal transduction, DNA replication, and repair. Enzyme characterization involves identifying, analyzing, and describing the functions, structures, kinetic properties, and roles of enzymes within biological systems. This characterization enables scientists to understand e......
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Disulfide bond mapping is a pivotal technique for precisely analyzing the connectivity and positions of disulfide bonds in proteins, crucial for understanding protein structure and function. Disulfide bonds are chemical links formed through the oxidation of thiol groups (-SH) on cysteine residues, contributing significantly to the stabilization of protein tertiary structures and the maintenance of protein activity. The process of disulfide bond mapping involves gradually breaking down proteins with di......
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• Protein-Protein Interaction Mapping Methods and Applications
Protein-protein interaction mapping provides a graphical framework to visualize the relationships and interactions among proteins. Proteins are fundamental to numerous biological processes, including structural maintenance, signal transduction, immune responses, and metabolism. However, individual proteins often cannot perform complex biological functions independently. Instead, they interact to form intricate networks that operate synergistically. As such, protein-protein interaction mapping has emer......
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A protein interaction map graphically represents the interaction relationships among proteins and serves as a vital tool in modern bioinformatics and systems biology research. Proteins, functioning as molecular machines responsible for most cellular activities, form complex networks through interactions. These networks participate in virtually all aspects of cellular life, including signal transduction, metabolic pathways, and gene expression regulation. Protein interaction maps aim to depict these in......
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