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Proteomics Databases
Metabolomics Databases
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LC-MS protein analysis is a cutting-edge technique that integrates the separation abilities of liquid chromatography (LC) with the detection and identification capabilities of mass spectrometry (MS) for protein research. This technology enables the separation and detection of proteins or peptides in samples to identify and quantify complex proteomes. Through such analysis, researchers can gain insights into disease pathogenesis, identify potential biomarkers, and support the early diagnosis and treatm......
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LC-MS-MS proteomics combines Liquid Chromatography (LC) with Tandem Mass Spectrometry (MS-MS) to facilitate comprehensive proteomic analysis. Initially, protein samples are enzymatically digested into peptides. These peptides are then separated using liquid chromatography and subsequently analyzed through tandem mass spectrometry. The resulting peptide mass data is compared against protein sequence databases to enable the qualitative and quantitative elucidation of proteins. This approach allows for a......
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• LC-MS-MS for Protein Identification
LC-MS-MS for protein identification is a highly efficient analytical method that integrates liquid chromatography (LC) and tandem mass spectrometry (MS-MS) to separate and identify proteins with exceptional precision. This approach is particularly powerful in identifying proteins within complex biological samples, leveraging the combined strengths of LC for separating peptides and MS-MS for their in-depth analysis. The methodology typically starts with protein extraction, enzymatic digestion into pept......
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• Analysis of Milk for the Lipids, Carbohydrates, and Proteins
The analysis of milk for the lipids, carbohydrates, and proteins constitutes a crucial area of research in food science and nutrition, because the proportions and structural forms of these three components not only influence the nutritional value of milk but also play a pivotal role in determining its stability and sensory attributes during processing and storage. Precise quantification and characterization of these components enable scientists and food manufacturers to refine milk processing techniqu......
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Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF) is a powerful analytical technique widely used for biomolecule and organic compound analysis. Its rapidity, simplicity, and precision have made it indispensable in modern life sciences research and clinical diagnostics. MALDI-TOF combines the gentle ionization provided by MALDI with the rapid and accurate mass measurement capabilities of TOF, enabling the detection and analysis of proteins, peptides, carbohydrates......
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MALDI protein analysis is a mass spectrometry technique employed for the detailed examination of proteins. This method involves blending the sample with a specific matrix substance, utilizing laser pulses for ionizing the sample molecules, and subsequently measuring their mass with a mass spectrometer. This approach facilitates the identification and analysis of complex protein molecules, contributing to a deeper comprehension of protein structures and functionalities. Proteins, as molecular machines,......
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• C-Terminal Amino Acid Analysis
C-terminal amino acid analysis is a technique employed to specifically recognize and identify the C-terminal (carboxyl-terminal) amino acid sequence in proteins or polypeptides. Unlike N-terminal amino acid analysis, which focuses on one end of the protein chain, C-terminal analysis centers on the opposite end, which is crucial for protein function, stability, and molecular interactions. In biological and biochemical studies, understanding the C-terminal structure of proteins can reveal their cellular......
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• Antibody Glycosylation Analysis
Antibody glycosylation analysis is a technique focused on examining the structural and functional characteristics of glycosylation within antibody molecules. As a prevalent form of post-translational modification, glycosylation is particularly significant in antibodies, which are pivotal immune proteins in biopharmaceuticals, disease diagnosis, and treatment. These modifications influence not only the structural integrity and stability of antibodies but also their biological activity, pharmacokinetics......
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• Tandem MS Peptide Sequencing
Tandem MS peptide sequencing is an advanced analytical technique utilized for elucidating protein amino acid sequences. This method integrates liquid chromatography and mass spectrometry to conduct multiple rounds of mass analysis on peptide fragments, thereby enabling precise sequencing within complex samples. In proteomics, tandem MS peptide sequencing is invaluable for providing primary structural information of proteins, such as amino acid sequences, which aids in elucidating the functions, intera......
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• Peptide Characterization Techniques
Peptide characterization techniques play a pivotal role in modern biological research and applications, encompassing fields such as proteomics, drug development, biomarker discovery, and disease diagnosis. In proteomics, these techniques are instrumental in analyzing peptides produced by protein degradation, facilitating the detailed characterization of protein structure and function. This understanding provides insights into the complex biochemical processes occurring within organisms. In drug develo......
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