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Proteomics Databases



Metabolomics Databases

• • How Single Cell Proteomics Accelerates Precision Medicine

  Precision medicine aims to formulate scientifically informed diagnostic and therapeutic strategies tailored to individual patient characteristics. This approach fundamentally depends on a deep understanding of disease mechanisms and the ability to monitor therapeutic responses dynamically. With the advancement of omics technologies, platforms such as genomic sequencing, transcriptome analysis, and single-cell RNA sequencing have laid the groundwork for precision medicine. However, precision medicine......

• • Single Cell Proteomics Strategies in Immunology Research

  The immune system exhibits a highly sophisticated capacity for dynamic regulation, playing pivotal roles in processes such as host defense, tumor surveillance, autoimmunity, and tissue repair. Emerging evidence indicates that immune cells display substantial functional heterogeneity depending on developmental stages, microenvironmental cues, or external stimuli. In recent years, advances in single-cell technologies have significantly enhanced the resolution at which immunological processes can be studied...

• • How to Efficiently Identify Protein Structures: Technologies and Tools Explained

  The three-dimensional structure of a protein is fundamental for understanding its biological functions, molecular mechanisms, and therapeutic potential. Compared to sequence analysis, protein structure determination provides greater explanatory power in elucidating functional mechanisms, mapping protein interaction networks, and facilitating drug discovery. Consequently, how to identify protein structures effectively has become a central challenge in contemporary life sciences. With advances in both .......

• • Optimized Workflow for Single Cell Proteomics Using Mass Spectrometry

  Single cell proteomics (SCP) represents one of the most innovative technologies in modern life sciences, enabling high-resolution analysis of protein expression and functional dynamics at the individual cell level. Particularly in highly heterogeneous systems such as tumors, the immune system, and stem cell populations, SCP offers a powerful approach to elucidate cell fate decisions, state transitions, and disease progression from a functional standpoint. Within the SCP experimental framework, the .......

• • Methods and Applications of Protein Circular Dichroism (CD) Spectroscopy

  Circular Dichroism (CD) spectroscopy is a chiroptical technique that detects the differential absorption of left- and right-circularly polarized light by chiral molecules. It has been widely employed to investigate protein secondary structure, conformational dynamics, thermal stability, and folding pathways. Owing to its non-destructive nature, high sensitivity, and ease of use, CD spectroscopy has become an essential tool for protein structural characterization. In the fields of life sciences and ......

• • How to Interpret High-Dimensional Data in Single Cell Proteomics

  Single cell proteomics (SCP) is rapidly emerging as a powerful tool for dissecting cellular heterogeneity and the dynamic nature of biological processes. By profiling protein expression at the resolution of individual cells, researchers can gain critical insights into the molecular underpinnings of tissue homeostasis, disease progression, immune responses, and more. However, SCP data are inherently high-dimensional, typically involving the quantification of hundreds to thousands of proteins per cell........

• • Integrating Single Cell Proteomics with scRNA-seq: Methods and Tools

  The rapid advancement of single-cell sequencing technologies has enabled researchers to dissect cellular heterogeneity at single-cell resolution. Among various approaches, single-cell RNA sequencing (scRNA-seq) primarily captures the transcriptomic landscape by profiling mRNA expression, whereas single cell proteomics (scProteomics) offers insights into protein-level expression, which more directly reflects functional cellular output. These two modalities represent distinct yet complementary dimension......

• • Using CD Spectroscopy to Evaluate Protein Thermal Stability

  Protein thermal stability is a key indicator of structural integrity and functional retention, and it is widely applied in basic research, protein engineering, and biopharmaceutical development. Circular Dichroism (CD) spectroscopy is a sensitive, rapid, and low–sample-volume technique commonly used to monitor conformational changes in protein secondary structure. It is particularly suitable for evaluating thermal denaturation behavior and thermal stability parameters such as the melting temperature .......

• • How to Analyze CD Spectra Using Deconvolution Algorithms

  Circular Dichroism (CD) spectroscopy is an important technique for investigating the secondary structures of proteins. However, a CD spectrum essentially represents the superposition of multiple structural components (such as α-helices, β-sheets, and random coils), and thus cannot directly reveal the proportion of each constituent. In such cases, the deconvolution algorithm serves as a crucial analytical approach. This study systematically presents the principles, workflow, and considerations for appl......

• • Circular Dichroism Analysis of Antibody Drugs

  In antibody drug development, structural characterization is a critical step to ensure both safety and therapeutic efficacy. Circular dichroism (CD) is an optical technique that relies on the differential absorption of left- and right-circularly polarized light by chiral molecules. Owing to its operational simplicity, high sensitivity, and minimal sample requirement, Circular Dichroism analysis has become a widely employed method for investigating protein secondary structures.   Introduction to Circul......