Resources
Proteomics Databases
Metabolomics Databases
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The protein pulldown assay is a widely employed in vitro technique for analyzing protein-protein interactions. By using a tagged bait protein to capture interacting partners, this method provides critical insights into protein relationships, binding affinity, and interaction specificity. Compared to yeast two-hybrid and co-immunoprecipitation assays, the protein pulldown assay offers direct and specific detection of interactions in a controlled environment. Its simplicity, high sensitivity, and abilit......
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Enzyme analysis involves investigating enzyme activity, kinetics, catalytic mechanisms, and regulatory effects (inhibition or activation) using a variety of experimental and technical approaches. Enzymes, which are protein or RNA molecules, act as biological catalysts, facilitating chemical reactions both intracellularly and extracellularly. They are often referred to as the "catalysts of life." The primary objective of enzyme analysis is to comprehensively understand enzymatic catalytic properties, r......
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• Transcriptomics and Proteomics
Transcriptomics and proteomics represent two complementary approaches for studying gene expression at different molecular levels. Transcriptomics focuses on RNA analysis to explore gene expression patterns, regulatory relationships, and the influence of environmental factors. In contrast, proteomics investigates the final gene expression products-proteins-examining their composition, structure, function, and interactions. As proteins directly execute cellular functions, proteomics offers critical ......
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• Overview of Full Amino Acid Profile
The full amino acid profile represents the qualitative and quantitative analysis of amino acids obtained by breaking down proteins into their individual amino acid components. This analysis reveals the amino acid composition of proteins and offers insights into their structure, function, and biological activity. The full amino acid profile is widely applied in proteomics, structural biology, and clinical diagnostics. By analyzing amino acid sequences and their proportions, researchers can est......
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Single-cell proteomics (SCP) represents a forefront area of biological research, dedicated to profiling protein expression patterns and their dynamics at the single-cell level. Traditional proteomics methods predominantly focus on population or tissue-scale analyses, limiting their capacity to resolve cellular heterogeneity. However, such heterogeneity is critical for understanding tissue functionality, biological mechanisms, and disease progression, underscoring the need for precise single-cell detec......
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• Introduction and Applications of CUT&Tag Technology
CUT&Tag (Cleavage Under Targets and Tagmentation) is an advanced technique designed for the study of DNA-protein interactions, primarily employed to identify the binding sites of transcription factors or histone modifications across the entire genome. The core principle of CUT&Tag involves the use of antibodies to specifically recognize target proteins or chromatin modifications, followed by the action of the Tn5 transposase, which generates sequencing-compatible tags at these binding sites. First......
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• The Hot Topic in Chromatin Biology: Insights into Histone Modification and CUT&Tag
Histone modifications are essential regulators of chromatin structure and gene expression, playing crucial roles in maintaining cellular function, genomic stability, and facilitating processes such as cell differentiation. Within the cell nucleus, DNA directs the synthesis of RNA and proteins, ensuring proper organismal function. DNA exists as a double helix and wraps around histones to form nucleosomes, which serve as the fundamental units of chromatin. Nucleosomes are organized into a bead-on-a-string...
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• Protein Characterization Services
Protein characterization services encompass analytical techniques designed to determine the physicochemical properties, structural features, and biological functions of proteins. As essential functional molecules in living organisms, proteins exhibit diverse structures and functions that underpin their critical roles in biological processes. Accurate protein characterization is vital for understanding protein behavior in cellular systems, tracking protein alterations in disease states, and identifying......
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Native mass spectrometry (native MS) is a biochemical research technique that enables scientists to analyze proteins without altering their native structure. This method's core advantage is its ability to maintain the protein's original conformation and functional state, ensuring more accurate and reliable data. Traditional denaturing mass spectrometry often involves disrupting the protein's native conformation to facilitate detection, which can lead to the loss of key structural information or altera......
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• Peptide Sequencing by Mass Spectrometry
Peptide sequencing by mass spectrometry is an analytical technique used to determine the amino acid sequence of proteins and identify their structural features. The core principle involves precise mass measurements of enzyme-digested protein fragments (peptides) using a mass spectrometer, enabling the deduction of the amino acid sequence of the peptide chain. This technology has wide-ranging applications. In basic research, it enables the identification and quantitative analysis of complex protein int......
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