Resources
Proteomics Databases
Metabolomics Databases
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• Co-Immunoprecipitation Mass Spectrometry
Co-immunoprecipitation mass spectrometry is a powerful technique used to investigate protein-protein interactions and the composition of protein complexes. Immunoprecipitation utilizes specific antibodies to capture and isolate target proteins along with their interacting partners. The success of this method depends on the specificity and affinity of the antibodies, enabling efficient enrichment of target proteins from complex biological samples. Mass spectrometry, known for its high sensitivity and r......
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Protein-Protein Interaction networks refer to complex networks formed by interactions between proteins, playing a crucial role in regulating biological processes. Within each cell, thousands of proteins do not exist in isolation but assemble through specific interactions to form molecular machines or regulatory networks, collectively driving essential biological functions. These networks consist of nodes (proteins) and edges (interactions between proteins) as their core elements, exhibiting highly dyn......
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Circular dichroism spectrum is a spectroscopic technique widely used to study the optical activity of chiral molecules. Chiral molecules, which cannot be superimposed on their mirror images, are commonly found in biological macromolecules such as proteins and nucleic acids. The circular dichroism spectrum measures the difference in absorption between left-handed and right-handed circularly polarized light (known as circular dichroism), providing valuable information on molecular configuration, conform......
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• Mass Spectrometry Protein Sequencing
Mass spectrometry protein sequencing is a core technology in modern biology, pharmacy, and medical research. It utilizes mass spectrometers to precisely analyze proteins, revealing their molecular structures, amino acid sequences, and modification states. As a powerful analytical tool, mass spectrometry protein sequencing offers several key advantages. Firstly, mass spectrometry has exceptional sensitivity, enabling the detection of extremely low-abundance proteins in complex biological samples. Secon......
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HCP analysis is a technique used to detect and quantify residual host cell proteins in biopharmaceutical production. Host cell proteins are byproducts produced alongside the target recombinant protein during its expression in host cells. The main goal of HCP analysis is to detect and quantify these proteins, ensuring the purity of biopharmaceutical products and reducing potential risks to human health. In particular, HCP contamination in the production of biopharmaceuticals can lead to adverse effects......
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Identification Protein refers to a series of experimental techniques used to identify and determine the types, quantities, functions, and modifications of proteins present in a sample. Proteins are essential mediators of various biological activities inside and outside the cell, and nearly all cellular functions require the involvement of proteins. Therefore, accurate identification protein, especially in complex biological samples, provides valuable information that aids scientists in understanding t......
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• Overview of Phosphoproteomics Analysis
Phosphoproteomics analysis is a pivotal technique for investigating protein phosphorylation and its regulatory roles in biological systems. As one of the most prevalent post-translational modifications, phosphorylation governs essential cellular processes, including signal transduction, metabolism, cell cycle progression, gene expression, and apoptosis. By systematically profiling phosphorylated proteins, researchers gain insights into cellular responses to external stimuli, regulatory mechanisms unde......
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TMT quantitative proteomics is a highly efficient protein quantification technique widely utilized in biological and medical research. By precisely quantifying protein abundance across samples, it reveals molecular changes within complex biological systems. This approach employs chemical labeling reagents to tag peptides from different samples with distinct isobaric mass tags, enabling simultaneous identification and quantification via mass spectrometry (MS). Compared to conventional protein quantific......
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• 2D-DIGE in Proteomics Analysis
Two-dimensional difference gel electrophoresis (2D-DIGE) is a widely employed technique in proteomics, designed to efficiently and accurately compare protein profiles across different samples. By combining two electrophoretic principles—Isoelectric Focusing (IEF) and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)—this method allows simultaneous analysis of multiple protein samples on the same gel, enabling high-resolution separation and quantitative analysis. 2D-DIGE is particula......
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Olink analysis is a high-throughput immunoassay platform technology designed for rapid and precise protein quantification. Leveraging Olink's unique Proximity Extension Assay (PEA) technology, researchers can simultaneously detect hundreds to thousands of protein biomarkers, significantly accelerating biomarker discovery and validation. PEA employs two antibodies that bind to the target protein, one carrying an extension probe. Upon binding, the probes form a complete DNA sequence, which is then detec......
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