Sequence and Mutation Analysis Service of Unknown Proteins or Peptides

In drug development and the production of biological products, it is essential to ensure that proteins are correctly folded and functional. Sequence mutations, whether from errors in DNA replication or incorrect amino acid incorporation during translation, can cause protein dysfunction or inactivation. Accurate identification and analysis of these sequence variations (SVs) are critical for maintaining the quality and efficacy of products. Our services are tailored for analyzing sequence and mutation in unknown proteins or peptides, utilizing cutting-edge mass spectrometry to thoroughly examine variations in the primary structure of proteins.

 



König, S. et al. Molecules. 2022.

 Figure 1. General Workflow for the Identification of Unknown Proteins of Interest

 

Services at MtoZ Biolabs

MtoZ Biolabs employs High-Performance Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS), combined with sophisticated bioinformatics analyses, to offer comprehensive de novo sequencing services. This technology can detect mutations at protein levels as low as 0.1%, uncovering even the most minute amino acid substitutions. Our approach integrates multiple enzymatic digestions and precision mass spectrometry to ensure exhaustive coverage and detailed analysis of protein sequences.

 

Service Advantages

1. Complete Sequence Coverage

Multiple digestions with a variety of proteases guarantee 100% coverage, precisely identifying each amino acid's position and any variations.

2. Advanced Sequencing Platform

Our platform merges High-Performance Liquid Chromatography-Ultraviolet-Tandem Mass Spectrometry (HPLC-UV/MS/MS) with advanced bioinformatics tools, ensuring accurate protein sequence interpretation.

3. Detailed Semi-Quantitative Analysis

We provide semi-quantitative assessments of mutations, offering insights into their specific effects.

4. High Sensitivity Detection

Capable of detecting mutations at protein levels down to 0.1%, our technology ensures comprehensive identification of all sequence variations.

5. Diverse Enzymatic Digestion Strategies

We use various proteases to produce a detailed peptide map, enhancing both sequence coverage and the accuracy of mutation detection.

6. Flexible Sample Processing

Our labs can handle diverse sample types, ranging from cell cultures and tissue samples to purified proteins.

7. Expert Bioinformatics Support

Our SIEVE software efficiently identifies all amino acid sequence variations within complex mixtures, providing detailed reports.

 

Applications

1. Protein Drug Development

We support the development of therapeutic proteins, ensuring their safety and effectiveness while optimizing drug design and production.

2. Novel Protein Identification 

Analyzes amino acid sequences of completely unknown new proteins, providing accurate sequence information to support basic research and new drug development.

3. Protein Quality Control

Before production, our cloning selection process helps screen out undesirable mutations, ensuring product consistency and quality.

4. Recombinant Biopharmaceuticals

Our analyses identify and quantify sequence variants in recombinant proteins, enhancing process optimization and product purity.

5. Functional Protein Research

By analyzing protein sequences and mutations, we reveal the relationship between protein function and structure, promoting advancements in life science research.

 

MtoZ Biolabs boasts a highly skilled and experienced research team dedicated to providing high-quality sequence and mutation analysis services to clients worldwide. Our technical platform, optimized and validated over many years, meets various complex sequence analysis needs. We offer customized service solutions to meet specific client requirements, ensuring high data accuracy and reproducibility. For more information about our sequence and mutation analysis services for unknown proteins or peptides, or for any technical inquiries, please visit our website or contact us via email. MtoZ Biolabs looks forward to collaborating with you to advance proteomics research and applications.