Should Positive Ion Mode or Negative Ion Mode Be Used for Amino Acid Mass Spectrometry Detection
Amino acid mass spectrometry detection is typically performed in positive ion mode. This preference arises from the propensity of amino acids to form cationic species in solution, particularly under acidic conditions. In mass spectrometric analysis, the positive ion mode allows for more efficient detection and characterization of these positively charged amino acid species.
Nevertheless, under certain conditions, negative ion mode may be employed—particularly when specific amino acids or their derivatives are more likely to form anionic species under the applied analytical conditions. Such cases are relatively uncommon and are largely determined by the amino acid's structural characteristics and the analytical objective.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
Related Services
Submit Inquiry
How to order?
