SUMO Modification Analysis Service

The Small Ubiquitin-like Modifier (SUMO) is an important post-translational modification that proteins undergo, involving the covalent attachment of a small, ubiquitin-like modifier to target proteins. SUMO proteins, typically about 100 amino acids in length and structurally similar to ubiquitin, have four main versions identified in mammals, from SUMO 1 to SUMO 4.

 

The process known as sumoylation is a critical and widespread post-translational modification in eukaryotic organisms that regulates various essential cellular functions. These include influencing the precise location of proteins within cells, modulating gene activity, participating in the composition of nuclear structures, combating viral invasion, and controlling cell cycle processes. The balance between sumoylation and its reverse process, desumoylation, is vital for understanding the physiological pathways and progression of many diseases. Research into this process can reveal disease mechanisms and aid in the development of new therapies and precision medicine.

 



Geiss-Friedlander, R. et al. Nat Rev Mol Cell Biol. 2007.

Figure 1. Protein Sumoylation Identification

 

Services at MtoZ Biolabs

MtoZ Biolabs utilizes advanced mass spectrometry technology and bioinformatics tools, combined with site-directed mutagenesis analysis, to provide high-precision sumoylation protein identification services. This includes enrichment of sumoylated peptides, identification of SUMO modification sites, structural analysis of SUMO modifications, mapping of sumoylated lysines, qualitative analysis of SUMO, and stability analysis of SUMO-modified proteins. Just tell us your needs and send your samples; MtoZ Biolabs handles everything from sample extraction to data analysis. Our technical team specializes in extracting and identifying sumoylated proteins from eukaryotic and prokaryotic organisms, using a combination of affinity chromatography and mass spectrometry to ensure precise and efficient operations.

 

Analysis Workflow

At MtoZ Biolabs, we combine state-of-the-art mass spectrometry with comprehensive bioinformatics to deliver precise sumoylation analysis. From enriching sumoylated peptides to mapping modification sites, our services are designed to provide deep insights into protein modifications:

1. Protein Extraction: Tailored to each sample type for optimal yield.

2. Enzymatic Digestion: Using specific enzymes to ensure complete protein coverage.

3. Targeted Enrichment: Focused isolation of sumoylation sites for detailed analysis.

4. Advanced Mass Spectrometry: High-resolution instruments provide detailed molecular insights.

5. Bioinformatics Analysis: Data is meticulously processed to pinpoint modification sites and understand structural impacts.

 

Service Advantages

1. Comprehensive Coverage

We thoroughly map sumoylation sites to reveal functional insights.

2. Professional Team

Our team of specialists ensures the highest standards of accuracy and reliability.

3. Efficiency

We offer rapid turnaround times, typically 5-7 days, with ongoing updates.

4. Tailored Solutions

Services are customized to meet the specific needs of each research project.

 

Sample Submission Requirements

1. Solid Samples: Minimum of 5 grams for plant tissue, 1 gram for animal tissue.

2. Liquid Samples: At least 1 milliliter of blood, 0.5 milliliters of serum, 10 milliliters of urine.

3. Cell and Microbial Samples: A minimum of 1×10^8 cells, or 400 milligrams dry weight for microbes.

4. Protein Samples: At least 1 milligram required.

 

MtoZ Biolabs is dedicated to enabling researchers to explore protein sumoylation's role in biological processes and disease. Contact us to learn more about how our services can support your research needs or to discuss a customized approach for your project.