Systemic Lupus Erythematosus Analysis Service

Systemic lupus erythematosus (SLE) is a classic systemic autoimmune disorder characterized by impaired autoimmune tolerance and high concentration of autoantibodies targeting self-antigens, which lead to organ damage. This pathological process involves multiple cytokines. Notably the role of CD40L (CD154) has been extensively studied in SLE, where CD40L is markedly upregulated. In both human and murine models, sustained CD40L expression in lupus T cells can drive excessive activation of B cells, contributing to the pathogenesis of disease. Elevated soluble CD30 levels in SLE serum mark for high disease activity. IL-10, a key Th2 cytokine, is implicated in this autoimmune dysregulation. Additionally, IL-13 has been a significant focus in SLE research. Increases in pro-inflammatory cytokines such as IL-6, IL-12, IL-18, and TNF-α can initiate the inflammatory process of SLE. These cytokines are found elevated expression in SLE patients and correlated with disease activity. It is well-documented that interferon-α levels are elevated in the serum of patients with active SLE, and this cytokine is responsible for the formation of vesicular inclusions in the renal endothelial cells of these patients.

 



Sarhan, D. et al. Int J Mol Sci. 2014.

Figure 1. Systemic Lupus Erythematosus - Flow Cytometric Cytokine Detection

 

MtoZ Biolabs employs flow cytometry for the simultaneous quantification of cytokines and proteins relevant to SLE, offering higher sensitivity and a broader detection range. The available cytokines for human samples are listed below and testing is also provided for other species. Free project evaluation!

 

Systemic Lupus Erythematosus - Indicators for Flow Cytometric Cytokine Detection

 



Table 1. Systemic Lupus Erythematosus - Human Sample Multifactor Flow Cytometry Kit

 

Table 2. Systemic Lupus Erythematosus - Human Sample Single Factor Flow Cytometry Kit

 

Sample Submission Requirements

1. Requires only 25 µL of Sample, Compared to 50 µL or 100 µL of Sample for ELISA Tests

2. Suitable for Use with Serum, Plasma, Cell Culture Supernatants, and Other Body Fluids

3. Samples of Body Fluids Should be Stored at -80°C and transported on Dry Ice

 

Deliverables

1. Experimental Procedures

2. Raw Data (Fluorescence Spectra)

3. Technical Report

4. Absolute Quantities of Each Assay Indicator (Excel Spreadsheets)