Targeted Metabolomics Analysis Service
Targeted metabolomics is a study in which defined metabolites in a sample are identified and quantitatively analyzed. Quantitation and semi-quantitation of defined metabolites are undertaken through the use of internal standard compounds. Targeted metabolomics has an advantage of high specificity and accuracy. Thus, this method has been widely used to analyze and compare multiple targeted metabolites under different physiological states, and is a critical analytical method for discovery of new biomarker in metabolic diseases and study of early diagnosis of diseases.
MtoZ Biolabs offers targeted metabolomics analysis service using an LC-MS-based multiple reaction monitoring (MRM) and GC-MS-based single ion monitoring (SIM) metabolomics platforms, which have characteristics of high accuracy, specificity, and sensitivity. We guarantee accurate analysis of targeted metabolites, even in low abundance. With our optimized sample preparation methods, interference from high-abundance dominant metabolites can be hugely reduced, thus further increase the detecting sensitivity.
Analysis Workflow
List of Targeted Metabolites Analysis Services
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Targeted Metabolomic Pathways |
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NO. |
Pathway |
NO. |
Pathway |
NO. |
Pathway |
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1 |
3 |
Lipid Metabolism (16) |
4 |
Glycan Biosynthesis and Metabolism (12) |
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2 |
Tryptophan Pathway (30) |
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Organic Compounds |
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NO. |
Substance |
NO. |
Substance |
NO. |
Substance |
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1 |
16 |
Energy Metabolism (83) |
31 |
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2 |
Adenosine Phosphate (3) |
17 |
32 |
Plant Lectins (4) |
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3 |
18 |
33 |
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4 |
Alkaloid (14) |
19 |
34 |
Purines (6) |
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5 |
20 |
Glucosinolate (5) |
35 |
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6 |
21 |
Lignans (4) |
36 |
Saponin (2) |
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7 |
22 |
Indoxyl Sulfate and p-Cresol Sulfate (9) |
37 |
Stilbenes (3) |
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8 |
23 |
38 |
Tannin (2) |
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9 |
Branched-Chain Keto Acids (3) |
24 |
Neurotransmitters (78) |
39 |
Terpenoid (21) |
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10 |
25 |
40 |
Toxin (4) |
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11 |
Catecholamines (6) |
26 |
Nucleic Acid modification (71) |
41 |
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12 |
27 |
Metabolic Flux (33) |
42 |
Trimethylamine Oxide (4) |
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13 |
Ceramides (50) |
28 |
43 |
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14 |
Chlorophyll (6) |
29 |
Oxidized Lipids (141) |
44 |
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15 |
Coumarin (3) |
30 |
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Inorganic Compounds |
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NO. |
Substance |
NO. |
Substance |
NO. |
Substance |
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1 |
Metal/Metallomics (69) |
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Sample Submission Requirements
1. Cells and Microbes Samples: 1×10^7cells or 100 μL/sample. Cellular activities should be terminated instantly, whereas maintaining the cell integrity.
2. Animal Tissues: 100 mg/sample. Collect soft tissues like brain, heart, liver, muscle, and skin. Immediately add preservative reagent and freeze at -80°C.
3. Plant Tissues: 200 mg/sample. Samples should be frozen in liquid nitrogen right after sample collection, and then transferred to -80°C for storage.
4. Serum Samples: Repeated freezing and thawing of sample must be eliminated. Serum samples should be settled down at room temperature for 30 min in the collection tube, and then transferred to centrifuge tube and centrifuged at 8,000 rpm, 5 min. After centrifugation, supernatant is aliquoted to freezing tubes with 200 uL/sample, and stored at -80°C.
5. Urine Samples: 2mL/sample. Urine samples can be aliquoted to centrifuge tubes with 2mL each tube, with addition 1/100 (w/v) sodium azide, and stored at -80°C.
6. Faeces Samples: 200 mg/sample.
Deliverables
1. Experiment Procedures
2. Parameters of Liquid Chromatography and Mass Spectrometer
3. MS Raw Data Files
4. MS Data Quality Checks
5. Metabolites Quantification Data
6. Bioinformatics Analysis (PCA, KEGG, etc.)
Related Services
Metabolomics
Quantitative Proteomics
Protein Analysis
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• Mycolic Acid Analysis Service
Mycolic acids (MAs) are distinctive markers of the cell envelope in Mycobacterium tuberculosis and related species. Their unique and complex structures can be extracted using specific organic solvents, such as esters of trehalose or glycerol, or by esterifying the terminal pentaarabinofuranosyl units of arabinogalactan (AG), form an insoluble polysaccharide-peptidoglycan matrix of the cell wall. Both forms are crucial for the atypical structure and impermeability of the cell envelope, contributing to ......
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• Leukotriene Analysis Service
Leukotrienes (LTs) are a group of inflammatory lipid mediators derived from arachidonic acid (AA) via the 5-lipoxygenase (5-LOX) pathway. A characteristic feature of LTs is that they are produced by leukocytes and share a conjugated triene structure. LTs consist of cysteinylleukotrienes (CysLTs) and leukotriene B4 (LTB4). CysLTs contain a cysteine ring, whereas LTB4 is a noncysteine containing dihydroxy-leukotriene. The subtypes of CysLTs include leukotriene C4 (LTC4), leukotriene D4 (LTD4), and leuko......
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• Glycosylated Hexosylceramides Analysis Service
Sphingolipids are fundamental structural components of cell membranes, forming lipid rafts by combining with other components. Sphingolipids are involved in regulating biological processes such as cell growth, differentiation, and apoptosis. Ceramide is the central hub of the sphingolipid pathway, including sphingomyelin, glucosylceramide, and galactosylceramide, and is mainly divided into hexosylceramides (HexCer), lactosylceramides (LacCer), sphingosine (Sph), dihydrosphingosine (DhSph),
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• Hydroxyeicosatetraenoic Acids (HETEs) Analysis Service
Arachidonic acid undergoes oxidation through several enzymatic pathways, including prostaglandin H2 synthase, 5-lipoxygenase, and cytochrome P450 enzymes-dependent epoxygenase reactions. These pathways utilize arachidonic acid as a substrate, leading to the production of various bioactive lipid mediators such as prostaglandins, thromboxanes, leukotrienes, and epoxyeicosatrienoic acids.
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• Cardiolipins Analysis Service
Cardiolipin (CL) consists of two phosphatidyl molecules. It is a tetra-acylated glycerophospholipid that plays crucial roles in both prokaryotic and eukaryotic cells. CL is found in membranes involved in creating electrochemical gradients, which aid in ATP production and substance transporting across the cell membrane. CL is abundant in the mitochondria of eukaryotic cells, particularly in the inner mitochondrial membrane, where it closely associates with cytochrome c oxidase, necessary for efficient ......
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• Sphingosine Profiling Service
Sphingosine (SPH) is a long-chain aliphatic compound with an acyclic carbon skeleton, typically featuring a 2-amino-1,3-diol functional group. The primary SPH in mammalian tissues are sphingosine (2S,3R-d-erythro-2-amino-1,3-octadec-4E-ene-diol), sphinganine (2S,3R-d-erythro-2-amino-1,3-octadecane diol), and 4-hydroxy-sphinganine (2S,3S,4R-erythro-2-amino-1,3,4-octadecanetriol). In yeast, the primary sphingoid bases are dihydrosphingosine and phytosphingosine.
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Wax esters are composed of a fatty alcohol molecule and a fatty acid. Organic acids usually contain a carboxyl group (-COOH), and alcohols have a hydroxyl group (-OH). When an organic acid combines with an alcohol, an ester is formed. In wax esters, the hydroxyl group of the fatty alcohol combines with the carboxyl group of the fatty acid to form an ester bond. There are various types of wax esters, mainly classified as saturated wax esters and unsaturated wax esters.
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• Polysaccharides Analysis Service
Polysaccharides are linked by the glycosidic bond of more than 10 monosaccharide molecules. They have relatively large molecular weights and are usually composed of hundreds to thousands of monosaccharide units. Nucleic acids, proteins, lipids, and polysaccharides are considered the four essential substances of life, playing crucial roles in numerous biological activities, including immune regulation, anti-tumor effects, reduction of blood glucose and blood lipids, antiviral activity,
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• Triglyceride Analysis Service
Triacylglycerols (TAGs) are esters derived from a glycerol and three fatty acid molecules. The simplest TAGs are those with three identical fatty acids. The length of the fatty acids of TAGs can range from C4 to C24 carbons, but the most common are C16, C18, and C20. Fatty acids can be saturated or unsaturated. Natural fatty acids found in plants and animals typically consist of even numbers of carbon atoms because they are biosynthesized from acetyl-CoA with two-carbon units.
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• Short-Chain Fatty Acids Analysis Service
Short chain fatty acids (SCFAs) are a subgroup of saturated aliphatic fatty acids containing 2-5 carbon atoms, with acetate (C2), propionate (C3), and butyrate (C4) being the most abundant. They are the final products of dietary fiber fermented by beneficial coliform bacteria. Humans lack enzymes capable of degrading large amounts of dietary fiber, which passes undigested through the upper gastrointestinal tract into the cecum and colon, where it is fermented by anaerobic microbiota.
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