Targeted Metabolomics Analysis Service
Targeted metabolomics is a study in which defined metabolites in a sample are identified and quantitatively analyzed. Quantitation and semi-quantitation of defined metabolites are undertaken through the use of internal standard compounds. Targeted metabolomics has an advantage of high specificity and accuracy. Thus, this method has been widely used to analyze and compare multiple targeted metabolites under different physiological states, and is a critical analytical method for discovery of new biomarker in metabolic diseases and study of early diagnosis of diseases.
MtoZ Biolabs offers targeted metabolomics analysis service using an LC-MS-based multiple reaction monitoring (MRM) and GC-MS-based single ion monitoring (SIM) metabolomics platforms, which have characteristics of high accuracy, specificity, and sensitivity. We guarantee accurate analysis of targeted metabolites, even in low abundance. With our optimized sample preparation methods, interference from high-abundance dominant metabolites can be hugely reduced, thus further increase the detecting sensitivity.
Analysis Workflow
List of Targeted Metabolites Analysis Services
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Targeted Metabolomic Pathways |
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NO. |
Pathway |
NO. |
Pathway |
NO. |
Pathway |
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1 |
3 |
Lipid Metabolism (16) |
4 |
Glycan Biosynthesis and Metabolism (12) |
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2 |
Tryptophan Pathway (30) |
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Organic Compounds |
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NO. |
Substance |
NO. |
Substance |
NO. |
Substance |
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1 |
16 |
Energy Metabolism (83) |
31 |
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2 |
Adenosine Phosphate (3) |
17 |
32 |
Plant Lectins (4) |
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3 |
18 |
33 |
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4 |
Alkaloid (14) |
19 |
34 |
Purines (6) |
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5 |
20 |
Glucosinolate (5) |
35 |
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6 |
21 |
Lignans (4) |
36 |
Saponin (2) |
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|
7 |
22 |
Indoxyl Sulfate and p-Cresol Sulfate (9) |
37 |
Stilbenes (3) |
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8 |
23 |
38 |
Tannin (2) |
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9 |
Branched-Chain Keto Acids (3) |
24 |
Neurotransmitters (78) |
39 |
Terpenoid (21) |
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10 |
25 |
40 |
Toxin (4) |
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11 |
Catecholamines (6) |
26 |
Nucleic Acid modification (71) |
41 |
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12 |
27 |
Metabolic Flux (33) |
42 |
Trimethylamine Oxide (4) |
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13 |
Ceramides (50) |
28 |
43 |
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14 |
Chlorophyll (6) |
29 |
Oxidized Lipids (141) |
44 |
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15 |
Coumarin (3) |
30 |
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Inorganic Compounds |
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NO. |
Substance |
NO. |
Substance |
NO. |
Substance |
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1 |
Metal/Metallomics (69) |
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Sample Submission Requirements
1. Cells and Microbes Samples: 1×10^7cells or 100 μL/sample. Cellular activities should be terminated instantly, whereas maintaining the cell integrity.
2. Animal Tissues: 100 mg/sample. Collect soft tissues like brain, heart, liver, muscle, and skin. Immediately add preservative reagent and freeze at -80°C.
3. Plant Tissues: 200 mg/sample. Samples should be frozen in liquid nitrogen right after sample collection, and then transferred to -80°C for storage.
4. Serum Samples: Repeated freezing and thawing of sample must be eliminated. Serum samples should be settled down at room temperature for 30 min in the collection tube, and then transferred to centrifuge tube and centrifuged at 8,000 rpm, 5 min. After centrifugation, supernatant is aliquoted to freezing tubes with 200 uL/sample, and stored at -80°C.
5. Urine Samples: 2mL/sample. Urine samples can be aliquoted to centrifuge tubes with 2mL each tube, with addition 1/100 (w/v) sodium azide, and stored at -80°C.
6. Faeces Samples: 200 mg/sample.
Deliverables
1. Experiment Procedures
2. Parameters of Liquid Chromatography and Mass Spectrometer
3. MS Raw Data Files
4. MS Data Quality Checks
5. Metabolites Quantification Data
6. Bioinformatics Analysis (PCA, KEGG, etc.)
Related Services
Metabolomics
Quantitative Proteomics
Protein Analysis
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• Sphingosine-1-Phosphate Analysis Service
Sphingosine-1-Phosphate (S1P) is a signaling sphingosine, which is the product of the phosphorylation of sphingosine by sphingosine kinase. S1P is dephosphorylated by sphingosine phosphatase to form sphingosine, and can be irreversibly degraded by sphingosine phosphate lyase. Many cells can secrete S1P, which then affects other cells in an autocrine or paracrine manner.
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• Polyprenols Analysis Service
Polyprenols are long-chain isoprenoid alcohols. The general formula for polyprenols is H-(C5H8)n-OH, where n refers to the number of isoprene units. Polyprenols are classified into three types: all-trans (solanesol-type), two-trans (Solanesol-type) and poly-cis (Betulaprenol-type), and three-trans and poly-cis (Ficaprenol-type). Despite their low content, polyprenols are natural bioregulators present in many plant tissues.
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• Glycosphingolipids Analysis Service
Glycosphingolipids are widely present in cell membranes and serum. They either exist in free form or are complexed with other proteins. The glycan head group determines the biological function of glycosphingolipids. Abnormal glycosylation of glycosphingolipids is closely related to various cancers, such as breast cancer, lung cancer, and brain tumors. Glycosphingolipid glycans are also involved in many cellular processes, such as signal transduction, receptor function, and cell differentiation.
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• Prostaglandin Analysis Service
Prostaglandins (PG) are a group of lipid compounds with hormone-like effects that exhibit diverse physiological activities in animals. PG are enzymatically derived from fatty acids with 20 carbon atoms and a 5-carbon ring. They belong to a subfamily of eicosanoids and prostanoid class of fatty acid derivatives. PG have two main derivatives: prostacyclin and thromboxane. Prostacyclin is a potent local vasodilator and inhibitor of blood platelet aggregation.
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• Glycerophospholipid Analysis Service
Glycerophospholipids are phospholipids based on glycerol, serving as the main components of biological membranes. The diversity of head groups, complex acyl chain combinations, and a broad range of ionization efficiencies pose significant challenges for the accurate quantification of glycerophospholipids. These compounds encompass hundreds of analytes commonly encountered in routine analysis and over a thousand species identified through repeated fragmentation scans of some samples.
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• Endocannabinoid Analysis Service
Endocannabinoids are amphipathilic molecules synthesized from phospholipids in the nervous system. They are a part of the endocannabinoid system, which includes G protein-coupled receptors. The most extensively studied endocannabinoids are N-arachidonoylethanolamine (anandamide) and 2-arachidonoylglycerol (2-AG).
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Eicosanoid, including prostaglandin (PG), thromboxane (TX), leukotriene (LT), and lipoxin (LX), is critical signaling molecule. Prostanoid, a subset of eicosanoids, comprises PG and TX. Prostanoids are named based on the number of carbon-carbon double bonds in their molecules. Most biologically active PGs and TXs belong to the series 2 molecules with two carbon-carbon double bonds.
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• Urine Eicosanoid Metabolites Analysis Service
Prostanoids, also known as eicosanoids, are a family of signaling molecules produced and secreted by various types of cells under both normal and pathological conditions. In the human body, these metabolites are excreted into body fluids such as plasma and urine. The precursors of prostanoids are 20-carbon polyunsaturated fatty acids (PUFAs) like arachidonic acid (C20:4), which are esterified in cell membrane phospholipids.
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• Diacylglycerol Analysis Service
Diacylglycerol (DAG), also known as diglyceride, consists of two fatty acid chains and a glycerol molecule. It primarily exists in two forms: 1,2-diacylglycerol and 1,3-diacylglycerol. DAG can be synthesized via the phosphatidic acid pathway in the endoplasmic reticulum by the action of phosphatidic acid phosphatase or produced during the lipolysis of triglycerides (TAG) by TAG lipase. DAG is also a crucial substrate for the biosynthesis of triglycerides, which are essential for energy storage,
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• Cholesteryl Ester Analysis Service
Cholesteryl esters derived from cholesterol, are dietary lipids. They form when enzymes in the human body metabolize cholesterol with an ester bond between the carboxyl group of fatty acids and the hydroxyl group of cholesterol. Cholesteryl esters are extensively found in animal body fluids (plasma lipoproteins) and can also appear as fatty streaks in blood vessel walls, contributing to atherosclerosis. Their strong hydrophobic nature results in low solubility in water.
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