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    Targeted Metabolomics Analysis Service

      Targeted metabolomics is a study in which defined metabolites in a sample are identified and quantitatively analyzed. Quantitation and semi-quantitation of defined metabolites are undertaken through the use of internal standard compounds. Targeted metabolomics has an advantage of high specificity and accuracy. Thus, this method has been widely used to analyze and compare multiple targeted metabolites under different physiological states, and is a critical analytical method for discovery of new biomarker in metabolic diseases and study of early diagnosis of diseases.

      MtoZ Biolabs offers targeted metabolomics analysis service using an LC-MS-based multiple reaction monitoring (MRM) and GC-MS-based single ion monitoring (SIM) metabolomics platforms, which have characteristics of high accuracy, specificity, and sensitivity. We guarantee accurate analysis of targeted metabolites, even in low abundance. With our optimized sample preparation methods, interference from high-abundance dominant metabolites can be hugely reduced, thus further increase the detecting sensitivity.

       

      Analysis Workflow

       

      MtoZ Biolabs offers targeted metabolomics analysis service using an LC-MS-based MRM and GC-MS-based SIM technologies, with high accuracy, specificity, and sensitivity.

       

      List of Targeted Metabolites Analysis Services

       

      Targeted Metabolomic Pathways Carbohydrates & Glycolysis Pathways    
      Organic Compounds Acyl CoAs Amino Acid Animal Hormones
      Aldehydes Bioamine Bile Acids
      Carnitines Carotenoids Fatty Acids
      Flavone Flax-Liganans Meat Biomarkers
      Nucleotides Oxysterols Organic Acids
      Plant Hormone Resveratrol Tryptophan Metabolites
      Thiols Vitamins & Coenzymes  
      Inorganic Compounds Metal/Metallomics Polyphenols  

       

      Sample Submission Requirements

      1. Cells and Microbes Samples: 1×10^7cellsor 100 μL/sample. Cellular activities should be terminated instantly, whereas maintaining the cell integrity.

       

      2. Animal Tissues: 100 mg/sample. Collect soft tissues like brain, heart, liver, muscle, and skin. Immediately add preservative reagent and freeze at -80°C.

       

      3. Plant Tissues: 200 mg/sample. Samples should be frozen in liquid nitrogen right after sample collection, and then transferred to -80°C for storage.

       

      4. Serum Samples: Repeated freezing and thawing of sample must be eliminated. Serum samples should be settled down at room temperature for 30 min in the collection tube, and then transferred to centrifuge tube and centrifuged at 8,000 rpm, 5 min. After centrifugation, supernatant is aliquoted to freezing tubes with 200 uL/sample, and stored at -80°C.

       

      5. Urine Samples: 2mL/sample. Urine samples can be aliquoted to centrifuge tubes with 2mL each tube, with addition 1/100 (w/v) sodium azide, and stored at -80°C.

       

      6. Faeces Samples: 200 mg/sample.

       

      Deliverables

      1. Experiment Procedures

      2. Parameters of Liquid Chromatography and Mass Spectrometer

      3. MS Raw Data Files

      4. MS Data Quality Checks

      5. Metabolites Quantification Data

      6. Bioinformatics Analysis (PCA, KEGG, etc.)

       

      Related Services

      Metabolomics

      Targeted Metabolomics

      Untargeted Metabolomics

      Lipidomics

       

      Quantitative Proteomics

      Label-Free

      iTRAQ/TMT

      SILAC/Dimethyl

      SWATH

      MRM

       

      Protein Analysis

      Protein Identification

      Protein Mass Measurement

      PTMs Identification

    • • Animal Hormones Analysis Service

      MtoZ Biolabs uses GC/MS and UPLC/TripleQuad5500 for animal hormone analysis, with high accuracy, specificity, and sensitivity.

    • • Bile Acids Analysis Service

      MtoZ Biolabs provides bile acids analysis service using UPLC/TripleQuad5500, which can quantitatively analyze over 90 kinds of bile acids.

    • • Bioamine Analysis Service

      MtoZ Biolabs offers targeted bioamine analysis service using UPLC/TripleQuad5500, with high accuracy, specificity, and sensitivity.

    • • Carbohydrates Analysis Service

      MtoZ Biolabs uses GC/MS and UPLC/TripleQuad5500 platforms for analyzing carbohydrates and glycolysis pathway metabolites, with high accuracy, specificity, and sensitivity.

    • • Carnitines Analysis Service

      MtoZ Biolabs uses UPLC/TripleQuad5500 for analyzing carnitine and derivatives, enabling highly accurate quantification of over 355 kinds of carnitines.

    • • Carotenoids Analysis Service

      MtoZ Biolabs offers targeted metabolomics analysis service using UPLC/TripleQuad5500, with high accuracy, specificity, and sensitivity.

    • • Fatty Acids Analysis Service

      MtoZ Biolabs offers targeted fatty acids analysis service using GC/MS, with high accuracy, specificity, and sensitivity.

    • • Flavones Analysis Service

      MtoZ Biolabs offers targeted flavones analysis service using UPLC/TripleQuad5500, with high accuracy, specificity, and sensitivity.

    • • Lignans Analysis Service

      MtoZ Biolabs offers targeted flax-lignans analysis service using UPLC/TripleQuad5500, with high accuracy, specificity, and sensitivity.

    • • Biomarkers Analysis Service

      MtoZ Biolabs offers meat biomarkers analysis service using UPLC/TripleQuad5500, with high accuracy, specificity, and sensitivity.

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