Targeted Metabolomics Analysis Service

Targeted metabolomics is a study in which defined metabolites in a sample are identified and quantitatively analyzed. Quantitation and semi-quantitation of defined metabolites are undertaken through the use of internal standard compounds. Targeted metabolomics has an advantage of high specificity and accuracy. Thus, this method has been widely used to analyze and compare multiple targeted metabolites under different physiological states, and is a critical analytical method for discovery of new biomarker in metabolic diseases and study of early diagnosis of diseases.



MtoZ Biolabs offers targeted metabolomics analysis service using an LC-MS-based multiple reaction monitoring (MRM) and GC-MS-based single ion monitoring (SIM) metabolomics platforms, which have characteristics of high accuracy, specificity, and sensitivity. We guarantee accurate analysis of targeted metabolites, even in low abundance. With our optimized sample preparation methods, interference from high-abundance dominant metabolites can be hugely reduced, thus further increase the detecting sensitivity.

 

Analysis Workflow

 



 

List of Targeted Metabolites Analysis Services

 

Targeted Metabolomic Pathways
NO.	Pathway	NO.	Pathway	NO.	Pathway
1	Carbohydrates & Glycolysis Pathways (158)	3	Lipid Metabolism (16)	4	Glycan Biosynthesis and Metabolism (12)
2	Tryptophan Pathway (30)
Organic Compounds
NO.	Substance	NO.	Substance	NO.	Substance
1	Acyl CoAs (6)	16	Energy Metabolism (83)	31	Plant Hormone (129)
2	Adenosine Phosphate (3)	17	Fatty Acids (74)	32	Plant Lectins (4)
3	Aldehydes (13)	18	Flavone (190)	33	Polyphenols (142)
4	Alkaloid (14)	19	Flax-Liganans (1)	34	Purines (6)
5	Amino Acid (125)	20	Glucosinolate (5)	35	Resveratrol (4)
6	Animal Hormones (13)	21	Lignans (4)	36	Saponin (2)
7	Bile Acids (158)	22	Indoxyl Sulfate and         p-Cresol Sulfate (9)	37	Stilbenes (3)
8	Bioamine (8)	23	Meat Biomarkers (7)	38	Tannin (2)
9	Branched-Chain Keto Acids (3)	24	Neurotransmitters (78)	39	Terpenoid (21)
10	Carotenoids (70)	25	Nucleotides (54)	40	Toxin (4)
11	Catecholamines (6)	26	Nucleic Acid modification (71)	41	Tryptophan Metabolites (30)
12	Carnitines (38)	27	Metabolic Flux (33)	42	Trimethylamine Oxide (4)
13	Ceramides (50)	28	Organic Acids (80)	43	Thiols (7)
14	Chlorophyll (6)	29	Oxidized Lipids (141)	44	Vitamin & Coenzymes (75)
15	Coumarin (3)	30	Oxysterols (54)
Inorganic Compounds
NO.	Substance	NO.	Substance	NO.	Substance
1	Metal/Metallomics (69)

 

Sample Submission Requirements

1. Cells and Microbes Samples: 1×10^7cells or 100 μL/sample. Cellular activities should be terminated instantly, whereas maintaining the cell integrity.

 

2. Animal Tissues: 100 mg/sample. Collect soft tissues like brain, heart, liver, muscle, and skin. Immediately add preservative reagent and freeze at -80°C.

 

3. Plant Tissues: 200 mg/sample. Samples should be frozen in liquid nitrogen right after sample collection, and then transferred to -80°C for storage.

 

4. Serum Samples: Repeated freezing and thawing of sample must be eliminated. Serum samples should be settled down at room temperature for 30 min in the collection tube, and then transferred to centrifuge tube and centrifuged at 8,000 rpm, 5 min. After centrifugation, supernatant is aliquoted to freezing tubes with 200 uL/sample, and stored at -80°C.

 

5. Urine Samples: 2mL/sample. Urine samples can be aliquoted to centrifuge tubes with 2mL each tube, with addition 1/100 (w/v) sodium azide, and stored at -80°C.

 

6. Faeces Samples: 200 mg/sample.

 

Deliverables

1. Experiment Procedures

2. Parameters of Liquid Chromatography and Mass Spectrometer

3. MS Raw Data Files

4. MS Data Quality Checks

5. Metabolites Quantification Data

6. Bioinformatics Analysis (PCA, KEGG, etc.)

 

Related Services

Metabolomics

Targeted Metabolomics

Untargeted Metabolomics

Lipidomics

 

Quantitative Proteomics

Label-Free

iTRAQ/TMT

SILAC/Dimethyl

SWATH

MRM

 

Protein Analysis

Protein Identification

Protein Mass Measurement

PTMs Identification

• • Animal Hormones Analysis Service

  MtoZ Biolabs uses GC/MS and UPLC/TripleQuad5500 for animal hormone analysis, with high accuracy, specificity, and sensitivity.

• • Bile Acids Analysis Service

  MtoZ Biolabs provides bile acids analysis service using UPLC/TripleQuad5500, which can quantitatively analyze over 90 kinds of bile acids.

• • Bioamine Analysis Service

  MtoZ Biolabs offers targeted bioamine analysis service using UPLC/TripleQuad5500, with high accuracy, specificity, and sensitivity.

• • Carbohydrates Analysis Service

  MtoZ Biolabs uses GC/MS and UPLC/TripleQuad5500 platforms for analyzing carbohydrates and glycolysis pathway metabolites, with high accuracy, specificity, and sensitivity.

• • Carnitines Analysis Service

  MtoZ Biolabs uses UPLC/TripleQuad5500 for analyzing carnitine and derivatives, enabling highly accurate quantification of over 355 kinds of carnitines.

• • Carotenoids Analysis Service

  MtoZ Biolabs offers targeted metabolomics analysis service using UPLC/TripleQuad5500, with high accuracy, specificity, and sensitivity.

• • Fatty Acids Analysis Service

  MtoZ Biolabs offers targeted fatty acids analysis service using GC/MS, with high accuracy, specificity, and sensitivity.

• • Flavones Analysis Service

  MtoZ Biolabs offers targeted flavones analysis service using UPLC/TripleQuad5500, with high accuracy, specificity, and sensitivity.

• • Lignans Analysis Service

  MtoZ Biolabs offers targeted flax-lignans analysis service using UPLC/TripleQuad5500, with high accuracy, specificity, and sensitivity.

• • Biomarkers Analysis Service

  MtoZ Biolabs offers meat biomarkers analysis service using UPLC/TripleQuad5500, with high accuracy, specificity, and sensitivity.