Top-Down Based Glycoprotein Analysis Service

Glycoproteins are proteins that are covalently bonded with one or more oligosaccharide chains. They are composed of a protein backbone and carbohydrate moieties. The glycan chains are attached via two main types of glycosylation: (1) N-glycosylation: Glycans are attached to the nitrogen atom in the side chain of asparagine residues through N-acetylglucosamine (GlcNAc); (2) O-glycosylation: Glycans are attached to the oxygen atom in the side chain of serine or threonine residues through N-acetylgalactosamine (GalNAc).Glycoproteins are prevalent in biological systems and fulfill a variety of biological roles, including cell recognition, signal transduction, and immune responses. The structural complexity and diversity of glycans necessitate detailed glycoprotein analysis for both scientific and clinical advancements.

Top-down glycoprotein analysis involves the direct examination of intact glycoproteins, bypassing the enzymatic digestion into smaller fragments. This technique preserves the glycoprotein's structural integrity, thus maintaining comprehensive information about the glycosylation patterns that might otherwise be lost or altered during digestion. This approach simplifies sample preparation and enhances efficiency while providing detailed structural insights, such as glycosylation sites, glycan structures, and protein sequences.

MtoZ Biolabs has developed a robust glycoprotein analysis platform leveraging high-throughput mass spectrometry (MS) technology. Our team consists of seasoned scientists and technical experts with extensive experience in clinical diagnosis and biomedical research. We are dedicated to offering high-quality and efficient services to support glycoprotein research and drug development. We provide free project evaluations and welcome inquiries for more details. Our technical specialists are available for complimentary business assessments.

Analysis Workflow

1. Sample Preparation

Extracting and purifying the target glycoprotein to ensure sample purity and stability.

2. Mass Spectrometry Analysis

Utilizing high-resolution mass spectrometers (e.g., LC-MS/MS) to analyze intact glycoproteins and acquire mass-to-charge ratio (m/z) data.

3. Data Processing

Employing specialized software for data analysis to identify glycosylation sites, glycan structures, and protein sequences.

4. Results Validation

Conducting experimental validation and repeat tests to confirm the accuracy and reliability of the analysis.

Service Advantages

1. Comprehensive Analysis

We provide extensive structural information on glycoproteins, including glycosylation sites, glycan structures, and protein sequences.

2. High Efficiency

Our streamlined processes enhance analysis efficiency and reduce turnaround times.

3. High Accuracy

Our high-resolution mass spectrometers and advanced data processing software ensure precise results.

4. Reliability

We provide rigorous validation protocols guaranteeing reliable and reproducible outcomes.

5. Expert Team

Our team of experienced scientists and technical experts delivers top-notch technical support and services.

Applications

1. Biomedical Research

Investigating the role of glycoproteins in diseases such as cancer, diabetes, and neurodegenerative disorders.

2. Drug Development

Facilitating the development and quality control of glycosylated therapeutics to ensure drug safety and efficacy.

3. Clinical Diagnosis

Detecting and diagnosing disease-associated glycoprotein biomarkers to aid in early diagnosis and personalized treatment strategies.