Ubiquitinated Protein Analysis Service

Ubiquitination involves the covalent attachment of ubiquitin molecules to target proteins, following these steps: (1) Activation: Ubiquitin-activating enzyme (E1) activates ubiquitin via ATP hydrolysis; (2) Conjugation: Ubiquitin-conjugating enzyme; (E2) transfers the activated ubiquitin to itself; (3) Ligation: Ubiquitin ligase (E3) identifies specific substrate proteins and attaches ubiquitin to the lysine residues of these proteins. This modification tags proteins for degradation, regulates their activity, alters their cellular localization, or affects their protein interactions. Ubiquitination is crucial in cell cycle regulation, signal transduction, and DNA repair. Abnormal ubiquitination is linked to diseases like cancer and neurodegenerative disorders, making its study significant for scientific and clinical advancements.

 

MtoZ Biolabs offers comprehensive Ubiquitinated Protein Analysis Service, leveraging advanced mass spectrometry to elucidate the formation, function, and biological roles of protein ubiquitination. Our expert team and cutting-edge equipment ensure high-quality results, supporting your research endeavors effectively. For more information on our ubiquitinated protein analysis service or any inquiries, please contact us. We look forward to collaborating with you and advancing scientific research together.

 

Analysis Workflow

1. Sample Preparation

Extracting and digesting proteins from cells or tissues.

 

2. Enrichment of Ubiquitinated Peptides

Utilizing anti-ubiquitin antibodies or affinity tags for peptide enrichment.

 

3. Mass Spectrometry Analysis

Performing LC-MS/MS to separate and identify enriched peptides.

 

4. Data Analysis

Identifying ubiquitination sites and patterns using databases and bioinformatics tools.

 



 

Service Advantages

1. High Sensitivity and Specificity

 Advanced mass spectrometry ensures efficient peptide enrichment and accurate identification.

 

2. Professional Team

Our experienced team provides comprehensive services from sample preparation to data analysis.

 

3. Customized Services

We offer tailored solutions and technical support to meet specific client needs.

 

4. Fast Delivery

We provide efficient processes and data analysis for timely, reliable results.

 

Applications

1. Cancer Research

Investigating ubiquitination pathway abnormalities in tumors to identify therapeutic targets.

 

2. Neuroscience

Studying ubiquitination's role in neuronal function and neurodegenerative diseases.

 

3. Cell Biology

Understanding ubiquitination's regulatory mechanisms in biological processes like the cell cycle and signaling.

 

4. Drug Development

Assessing drug effects on protein ubiquitination and identifying new drug targets.

 

Sample Submission Requirements

 



For more sample details, please consult our technical team.

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Ubiquitinated Protein Analysis

4. Mass Spectrometry Images

5. Raw Data