Unknown Protein Sequence Analysis Service

Analyzing unknown protein sequences is a critical area in contemporary biological research. Through the examination of amino acid sequences of unknown proteins, scientists can uncover the functions, structures, and interactions of these proteins. Understanding protein sequences is pivotal for disease research, drug discovery, and fundamental biological studies, fostering scientific progress and technological innovation.

 

MtoZ Biolabs offers comprehensive services for unknown protein sequence analysis, providing high-quality data and in-depth insights into the roles of unknown proteins in biological processes. These services support advancements in both research and clinical applications. We offer free project evaluations and encourage you to learn more about our offerings. Our technical specialists are available for a complimentary business assessment.

 

Analysis Workflow

1. Sample Preparation

(1) Extracting the target protein sample, typically requiring purification steps to eliminate other proteins and contaminants.

(2) Using appropriate buffers and reagents to maintain protein stability and functionality.

 

2. Protein Digestion

(1) Employing proteases (e.g., trypsin) to cleave the protein sample into smaller peptides.

(2) Further purifying the digested sample to remove undigested proteins and other impurities.

 

3. Mass Spectrometry Analysis

(1) Analyzing the digested peptides using techniques such as liquid chromatography-mass spectrometry (LC-MS) or matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS).

(2) Acquiring mass spectrometry data of the peptides, typically including mass-to-charge ratio (m/z) and relative abundance.

 

4. Data Processing

(1) Utilizing specialized software for processing and analyzing the mass spectrometry data.

(2) Generating potential amino acid sequences from the peptide mass spectra.

 

5. Database Search

(1) Comparing the generated peptide sequences with known protein databases (e.g., UniProt, NCBI).

(2) Identifying matching protein sequences or perform homology searches.

 

6. Protein Identification

(1) Based on database search results, identifying the target protein sequence.

(2) Comparing and analyzing possible post-translational modifications (PTMs) such as phosphorylation and glycosylation.

 

7. Result Verification

Confirming the accuracy of the protein sequence using additional experimental methods such as mass spectrometry verification, Western blotting, or manual interpretation of protein mass spectra.

 

8. Data Analysis and Reporting

Compiling and analyzing the identification results to determine the protein's function and characteristics.

 

Applications

1. Revealing New Biological Functions and Mechanisms

By analyzing unknown protein sequences, novel proteins and their functions can be discovered, shedding light on previously unexplored biological processes and mechanisms. This provides new perspectives and data for fundamental biological research.

 

2. Disease Research

Unknown proteins may be intimately linked to the development and progression of diseases. Analyzing these proteins can lead to the discovery of new disease markers and pathogenic mechanisms, offering new targets and strategies for disease diagnosis, prevention, and treatment.

 

3. New Drug Development

Understanding the sequences and functions of unknown proteins can aid in identifying new drug targets, thereby accelerating drug development. This is particularly valuable in precision medicine, where new protein targets can result in more effective treatments.

 

4. Biomarker Discovery

Unknown proteins may serve as biomarkers for specific diseases or physiological conditions. Detecting these biomarkers can facilitate early diagnosis and prognosis evaluation. In complex diseases such as cancer and cardiovascular disorders, new biomarkers can significantly enhance diagnostic accuracy and treatment efficacy.

 

Service Advantages

1. Comprehensive proteome mapping (complete sequence identification).

2. Advanced data analysis techniques.

3. Exceptional precision and accuracy.

4. Rigorous sequence prediction and stringent validation protocols.

5. Minimal sample requirement (100 μg).

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Unknown Protein Sequence Analysis

4. Mass Spectrometry Images

5. Raw Data