Verification Service of Synthetic Peptide Sequence

With the rapid advancements in biotechnology and bioengineering, the synthesis and application of peptides have become increasingly prevalent. Peptides are extensively used in cancer diagnosis and treatment, antibiotic drug development, epitope mapping, antibody production, and vaccine design. Compared to small molecule drugs, peptides exhibit higher potential in terms of efficacy, safety, and tolerability, gaining attention as promising drug candidates for various diseases.

Synthetic peptide sequence verification involves determining the amino acid sequence of the peptide chain, which is essential for ensuring the accuracy and functionality of synthetic peptides. Edman degradation is a classic method for peptide sequencing, capable of sequentially revealing the N-terminal amino acid sequence without disrupting other peptide bonds. This method is suitable for both natural and non-natural α-amino acid residues at the N-terminus, as long as their α-amino group is unprotected. The efficiency of Edman degradation lies in the high yield of amino acid-PTH formation, making it ideal for identification using chromatography, electrophoresis, or mass spectrometry.In some cases, the structural complexity or length of the synthetic peptide may not be suitable for Edman degradation. For synthetic peptides with more than 70 amino acid residues, we employ protease digestion to break them down into smaller fragments, followed by sequence analysis of each fragment. This method complements Edman degradation, ensuring comprehensive and accurate sequence verification.

MtoZ Biolabs provides professional synthetic peptide sequence verification services to accelerate your peptide projects. Our highly skilled team collaborates closely with clients to address research challenges. If you are interested in our services, please contact us. We look forward to working with you.

Analysis Workflow

1. Sample Preparation

(1) Sample dissolution: Dissolving the synthetic peptide in an appropriate solvent (e.g., water, methanol, or acetonitrile).
(2) Sample concentration determination: Measuring the concentration of the peptide solution to ensure accurate analysis.

2. Mass Spectrometry Analysis

(1) Instrument selection: Using a mass spectrometer (e.g., MALDI-TOF or ESI-MS) for preliminary peptide mass analysis.
(2) Data acquisition: Obtaining mass spectrometry data for the peptide, including molecular ion peaks and fragment ion peaks.

3. Data Analysis

(1) Molecular weight confirmation: Confirming the molecular weight of the synthetic peptide using mass spectrometry data to see if it matches the theoretical molecular weight.
(2) Fragment ion analysis: Performing MS/MS fragment ion analysis to verify the amino acid sequence of the peptide chain.

4. Liquid Chromatography Separation

(1) HPLC analysis: Using HPLC for purity analysis and separation of the peptide.
(2) Chromatogram interpretation: Analyzing the chromatogram to confirm the purity and retention time of the peptide compared to the standard.

5. Sequence Confirmation

(1) Tandem mass spectrometry (MS/MS): Performing tandem mass spectrometry analysis on peptide fragments to further confirm the sequence.
(2) Database comparison: Comparing the obtained mass spectrometry data with theoretical sequences in a database to confirm the amino acid sequence of the peptide.

6. Purity Determination

(1) UV absorption: Using UV absorption methods to determine the purity of the peptide.
(2) Quality assessment: Evaluating the overall purity and quality of the peptide by combining HPLC and mass spectrometry data.

7. Final Report

(1) Data compilation: Compiling all experimental data and analysis results.
(2) Report generation: Generating a detailed report including sample information, analysis methods, experimental results, and conclusions.

Applications

1. Drug Development

In drug development, synthetic peptides are often used as drug candidates. Verifying their sequences ensures the accuracy of their function and mechanism of action studies.

2. Biomarker Research

Synthetic peptides can be used as biomarkers for disease diagnosis and monitoring. Verifying their sequences ensures the reliability of the detection results.

3. Vaccine Development

In vaccine development, synthetic peptides are used as antigens. Verifying their sequences ensures the effectiveness and safety of the vaccine.

4. Protein-Protein Interaction Studies

Synthetic peptides are used to study protein interactions. Verifying their sequences ensures the precision of the research.